Molecular genetics 2600-OG-EN-MG

Laboratory: Isolation of genomic DNA from oilseed rape. Restriction digestion of the gDNA with enzymes of varying sensitivity towards methylation. Preparation of non-isotope-labeled molecular probe. Electrophoresis of the digested DNA and its capillary transfer from the gel to a membrane. Southern hybridization. Visualization of hybridization by means of a colorimetric method. DNA sulfonation. Amplification of the sulfonated DNA. Summary.

If it is necessary to conduct classes remotely, the practical elements will be presented in the form of recordings and/or photos with their detailed description. The theoretical part of it will be presented in the form of a presentation.

Całkowity nakład pracy studenta

Contact hours with teacher: - participation in laboratory- 30 hrs - consultations- 12 hrs Self-study hours: - preparation for laboratory- 8 hrs - writing report in a form of an article- 15 hrs - reading literature- 20 hrs - preparation for exam- 15 hrs Altogether: 100 hrs (4 ECTS)

Efekty uczenia się - wiedza

Student W1: has advanced knowledge of genetics, biology and molecular biology (K_W01, K_W02, K_W03) W2: characterizes types of epigenetic mechanisms, their origin and importance for cell metabolism (K_W01, K_W02, K_W03) W3: knows bioinformatics systems and tools used to study DNA methylation (K_W12, K_W13) W4: knows techniques used to analyze DNA methylation (K_W05)

Efekty uczenia się - umiejętności

Student U1: is able to plan and carry out experiments, applying several methods, aiming at analyzing the level of DaNA methylation (K_U01, K_U06, K_U07, K_U13) U2: is capable of interpreting obtained experimental results (K) U11, K_U13) U3: can use bioinformatic methods to identify CpG islands (K_U09)

Efekty uczenia się - kompetencje społeczne

Student K1: continually improves the level of his knowledge(K_K01, K_K04) K2: is aware of copyrights, especially when preparing presentations (K_K05) K3: maintains substantive and critical assessment of own work (K_K07) K4: works in accordance with the principles of fair and secure cooperation in the implementation of a joint project (K_K02, K_K03, K_K09)

Metody dydaktyczne

Expository teaching methods: - experimental, practical, laboratory If it is necessary to conduct classes remotely, the practical elements will be presented in the form of recordings and/or photos with their detailed description. The theoretical part of it will be presented in the form of a presentation.

Metody dydaktyczne poszukujące

- ćwiczeniowa
- doświadczeń
- laboratoryjna

Rodzaj przedmiotu

przedmiot fakultatywny

Wymagania wstępne

Basic courses of cell biology, genetics and molecular biology

Koordynatorzy przedmiotu

Justyna Boniecka

Kryteria oceniania

Assessment methods:

- final written exam (70% of the final mark)

- report in a form of an article (15% of the final mark)

- activity (15% of the final mark)

Assessment criteria:

satisfactory - 60%

satisfactory plus - 68%

good - 76%

good plus - 84%

very good - 92%

Exam for this course can possibly be done using online available tools.

Literatura

Brown TA 2018 Genomes. Nicoglou A, Merlin F. 2017 Epigenetics: A way to bridge the gap between biological fields. Stud Hist Philos Biol Biomed Sci 66:73-82. Huang B, Jiang C, Zhang R. 2014 Epigenetics: the language of the cell? Epigenomics 6:73-88.

Więcej informacji

Dodatkowe informacje (np. o kalendarzu rejestracji, prowadzących zajęcia, lokalizacji i terminach zajęć) mogą być dostępne w serwisie USOSweb:

Strona przedmiotu 2600-OG-EN-MG w USOSweb