Biologia molekularna 1655-Lek11BMOLE-J

In the first semester, students will delve into "Molecular Biology" through a comprehensive curriculum comprising 20 hours of lectures and 20 hours of tutorials. This course aims to provide students with a solid understanding of the intricate molecular processes underlying human biology. By exploring topics such as DNA replication, gene expression, protein synthesis, and molecular signaling pathways, students will gain foundational knowledge essential for comprehending the complexities of clinical subjects. Mastery of these molecular principles is crucial for students' daily professional practice, as it forms the basis for diagnosing and treating various diseases, interpreting laboratory results, and developing innovative therapeutic interventions.
In the first semester, students will delve into "Molecular Biology" through a comprehensive curriculum comprising 20 hours of lectures and 20 hours of tutorials. This course aims to provide students with a solid understanding of the intricate molecular processes underlying human biology. By exploring topics such as DNA replication, gene expression, protein synthesis, and molecular signaling pathways, students will gain foundational knowledge essential for comprehending the complexities of clinical subjects. Mastery of these molecular principles is crucial for students' daily professional practice, as it forms the basis for diagnosing and treating various diseases, interpreting laboratory results, and developing innovative therapeutic interventions.
The lecture series will cover a diverse array of topics, including the variability and mutation of genes, chromosomal mutations, and the mechanisms of DNA repair. Students will explore further the structure and functions of DNA and RNA, as well as the organization of prokaryotic and eukaryotic chromosomes. The complexity of the genome and the flow of genetic information will also be thoroughly explored. Additionally, lectures will delve into gene manipulation techniques such as vectors, cloning, restriction enzymes, and the regulation of gene expression. The sessions will also touch upon topics like the normal human karyotype, genetic disorders, genetic engineering, and various methods employed in molecular biology research, including the regulation of prokaryotic transcription.
Complementing these lectures, tutorials will provide hands-on experience and practical skills essential for molecular biology research. Through a series of guided exercises, students will delve into topics such as the regulation of gene expression and the identification of Barr bodies in epithelial cells. They will learn techniques for DNA isolation from biological samples, as well as the evaluation of isolated DNA using agarose gel electrophoresis. Additionally, tutorials will cover RNA isolation, quantification, and quality assessment of nucleic acids, as well as electrophoretic separation techniques and nucleic acid amplification using PCR.
By providing both theoretical knowledge and practical skills, these "Molecular Biology" classes aim to empower students to navigate the complexities of clinical subjects with a deep understanding of the molecular mechanisms underpinning human physiology.