Molecular genetics in veterinary science 7404-WET-GEN

1. Introduction to molecular genetics (molecules, methods, problems to solve);
2. Setup of the molecular genetics laboratory (what is used and why), good practices in molecular laboratory;
3. DNA – the molecule characteristics and extraction methods;
4. RNA – the molecule characteristics and extraction methods;
5. Assessment of the concentration and structure of DNA and RNA samples (gel electrophoresis and fluorescence);
6. Introduction to PCR and qPCR;
7. The rules and tools for oligonucleotide primer design and primer handling;
8. Analysis and interpretation of qPCR data (absolute quantification);
9. Analysis and interpretation of qPCR data (relative quantification);
10. Genotyping in principle;
11. Genotyping methods to determine heritable diseases;
12. Sequencing methods and library preparation;
13. Sequence alignment methods (BLAST);
14. Basics of bioinformatics (introduction to R);
15. Case studies in veterinary medicine – students’ presentations.

Total student workload

Contact hours with teacher: - participation in lectures - 30 hrs - consultations- 2 hrs Self-study hours: - preparing presentation- 13 hrs - reading literature- 15 hrs - preparation for test- 15 hrs Altogether: 75 hrs (3 ECTS)

Learning outcomes - knowledge

Student W1: Knows and understands the main development trends of molecular genetics in veterinary sciences P8S_WG; W2: Knows and understands the modern methodology of scientific research in the field of molecular genetics in veterinary sciences P8S_WG; W3: Knows and understands the problems and dilemmas related to research in the field of molecular genetics in veterinary sciences P8S_WG, P8S_WK;

Learning outcomes - skills

Student U1: Is able to collect literature on molecular genetics research applied in veterinary sciences and critically analyze it P8S_UW; U2: Is able to fully summarize the results of other people's work on the application of molecular genetics in veterinary sciences and present them in the form of an oral presentation P8S_UK; U3: Thanks to good knowledge of English, is able to formulate and present their own paper on molecular genetics and understand someone else's P8S_UK, P8S_UO.

Learning outcomes - social competencies

Student K1: Is ready to develop research in the field of molecular genetics in veterinary sciences P8S_KR; K2: Is ready to take a critical attitude towards the achievements in the field of molecular genetics in veterinary research P8S_KK.

Teaching methods

Informative lecture, participatory lecture, problem-based lecture

Expository teaching methods

- informative (conventional) lecture
- problem-based lecture
- participatory lecture

Type of course

compulsory course

Prerequisites

None

Course coordinators

Anna Sławińska

Assessment criteria

Assessment methods
- Test W1, W2
- Presentation W3, U1, U2, U3, K3
- Active participation in classes U3, K1
Assessment criteria:
- Exam: 60% of the final grade
- Presentation: 40% of the final grade
The exam contains 10 open questions, scoring 1 point per question, minimum number of points to pass: 6 points. The presentation is assessed based on the total number of points awarded for: substantive value (10 points), comprehensible message (10 points) and answers to questions (5 points). Maximum number of points for the presentation: 25 points, minimum number of points needed to pass: 15 points.
Assessment of the colloquium and presentation (% of maximum points):
Unsatisfactory < 60%
sufficient 60-70%
sufficient plus 71-80%
good 81-87%
good plus 88-94%
very good >94%
In the event of receiving an unsatisfactory grade for the exam and presentation, it is possible to retake it, which is carried out on the same principles.

Practical placement

not applicable

Bibliography

F.W. Nicholas. Introduction to veterinary genetics. Wiley-Blackwell 2010
M. Świtoński. Genetyka ogólna i weterynaryjna. PWN, 2023
Scientific publications and databases (np., NCBI, Pubmed)

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 7404-WET-GEN in USOSweb