Chromatographic methods in veterinary science 7404-WET-CHRO

1. The general principles of chromatography. Types of chromatographic techniques.
2. Planar chromatography.
3. Gas chromatography.
4. Liquid chromatography.
5. Seperation, retention and resolution. Stationary and mobile phases.
6. Detectors in chromatography. Sensitivity and selectivity.
7. LC-MS/MS.
8. Target and non-target approaches. Metabolomics.
9. LC-MS/MS based proteomics.
10. Steps of method optimization.
11. Sample preparation.
12. Method validation.
13-15. Discussion of students’ projects.

Total student workload

Hours conducted with the participation of instructors: - lectures: 30 h Time devoted to individual student work (45 hours): - preparation for classes: 20 hours - preparation of final paper: 20 hours. - participation in consultations: 5 hours Total: 75 hours (3 ECTS)

Learning outcomes - knowledge

W1 – the PhD student understands theoretical background of different chromatographic techniques - P8S_WG W2 – the PhD student knows potential application of chromatographic techniques in veterinary sciences - P8S_WG

Learning outcomes - skills

U1 – the PhD student can assess advantages and disadvantages of specific chromatographic techniques in particular applications - P8S_UW U2 – the PhD student can critically interpret the results of studies in veterinary science using chromatographic techniques - P8S_UK, P8S_UW U3 – the PhD student can design the experiment using chromatographic techniques, including method optimization and validation - P8S_UO, P8S_UW

Learning outcomes - social competencies

K1 – the PhD student is critical of their accomplishments - P8S_KK K2 – the PhD student understands and applies in their research ethical rules related to veterinary sciences - P8S_KO

Teaching methods

- lecture, presentation - discussion, analysis and problem solving

Expository teaching methods

- informative (conventional) lecture
- participatory lecture
- problem-based lecture

Exploratory teaching methods

- case study
- classic problem-solving

Type of course

elective course

Prerequisites

Knowledge of chemistry and biology at 2nd level university studies

Course coordinators

Małgorzata Olejnik

Assessment criteria

Project presentation: case study of application of chromatography in veterinary science based on own research or set of scientific papers
Assessment criteria:
- presentation of the clinical/ scientific background 15%
- proper experiment design, including justification of chromatography as analytical technique– 25%
- presentation of method development and validation plan – 25%
- critical interpretation of results – 25%
- discussion – response to the questions from the group and the lecturer – 10%

Practical placement

Not applicable

Bibliography

Set of publications will be made available to students gradually during classes.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 7404-WET-CHRO in USOSweb