Conducted in terms: 2022/23Z, 2023/24Z, 2024/25Z, 2025/26Z

ISCED code: 0511

ECTS credits: 1

Language: Polish

Organized by: Faculty of Biological and Veterinary Sciences

Molecular diagnostic tests 2600-S1-CM-MTD

Laboratory classes are devoted to diagnostic techniques using the analysis of nucleic acids and proteins. The following content is covered during the classes:
- validation of analytical and diagnostic methods based on modern diagnostic and research methods,
- analysis of the structure of nucleic acids and basic methods of their isolation, qualitative and quantitative analysis,
- use of polymerase chain reaction (PCR),
- use of basic research methods using antibodies.

Term 2022/23Z:

None

Term 2023/24Z:

None

Term 2024/25Z:

None

Term 2025/26Z:

None

Total student workload

- participation in laboratory exercises: 20 hours - consultations: 4 hours - preparation for laboratory exercises and final test: 4 hours - own work on project, term paper, report, paper or presentation: 2 hours Total: 30 hours

Learning outcomes - knowledge

Student: W1 - knows methods of detecting molecules (nucleic acids and proteins) in biological material, the expression of which can be used as markers of disease lesions, knows the basic principles of working with biological material, K_W03, K_W04, K_W09 W2 - has knowledge of selected diagnostic methods, knows the principles of validation of analytical and diagnostic methods based on modern diagnostic and research methods, K_W03, K_W04, W3 - knows the genetic determinants of diseases, K_W08, K_W10 W4 - is aware of the scale of the social problem of genetically determined diseases, knows the basic molecular methods used to identify them, K_W10.

Learning outcomes - skills

Student: U1 - demonstrates the ability to read and understand professional literature in the native language and English - K_U03, K_U09 U2 - has the ability to isolate and determine molecules useful in the diagnosis of human diseases, including DNA/RNA isolation using columns and phenol-based isolation, preparing samples for FCM analysis - K_U04, K_U01, K_U03, K_U05 U3 – has the ability to interpret results obtained using techniques analyzing cell/tissue molecules - K_U05, K_U04, K_U08, K_U09

Learning outcomes - social competencies

Student: K1 - knows modern diagnostic methods and at the same time their limitations and resulting consequences, K_K04, K_K06 K2 - is aware of the need to comply with ethical principles, K_K05 K3 - has the ability to organize individual and team work, K_K02, K_K06 K4 - is aware of the social scale of the risk of diseases, including genetically determined diseases and cancer, and understands the importance of molecular diagnostics of these diseases, K_K04, K_K06

Course coordinators

Term 2024/25Z:

Anna Brożyna

Term 2023/24Z:

Anna Brożyna

Term 2025/26Z:

Anna Brożyna

Term 2022/23Z:

Kinga Linowiecka
Justyna Szpotan

Teaching methods

Methods - exercise - experiences - laboratory - observations

Observation/demonstration teaching methods

- display

Expository teaching methods

- description

Exploratory teaching methods

- practical
- presentation of a paper
- experimental
- project work
- observation

Type of course

compulsory course

Prerequisites

Basic knowledge in the field of biology, cell biology, genetics and biochemistry regarding the structure and function of nucleic acids, human genes and types, gene expression, mutations, mutagenic factors. Basic information on the methods of inheritance of monogenic diseases.

Assessment criteria

1. Written control tests covering specific topics covered during classes;
2. Assessment of a multimedia presentation prepared and presented by the student on a specific topic given by the instructor.
3. Continuous assessment (ongoing preparation of students for classes and their activity).

Assessment of colloquiums is based on the points obtained by the student, according to the following scale:

very good - 94-100%
db+ - 86-93%
db - 77-85%
dst+ - 69-76%
dst - 61-68%

A necessary condition for passing the exercises is also 100% attendance. In case of absence, the student is obliged to prepare a presentation on the topic previously agreed with the lecturer.

Practical placement

n/a

Bibliography

1. Bal J. Biologia molekularna w medycynie. Elementy genetyki klinicznej. Warszawa: PWN..
2. Epstein RJ. Biologia molekularna człowieka. Lublin: Czelej.
3. Korf BR. Genetyka człowieka rozwiązywania problemów medycznych. Warszawa: PWN.
4. Buchowicz J. Biotechnologia molekularna. Warszawa: PWN.
5. Lewiński A, Liberski P, red. Biologia molekularna człowieka. Lublin: Czelej.
6. Drewa G, Ferenc T. Podstawy genetyki dla studentów i lekarzy. Red. Wydawnictwo Medyczne Urban & Partner, Wrocław.
7. Szala S.Terapia genowa. Wydawnictwo Naukowe PWN

Term 2022/23Z:

None

Term 2023/24Z:

None

Term 2024/25Z:

None

Term 2025/26Z:

None

Notes

Term 2022/23Z:

None

Term 2023/24Z:

None

Term 2024/25Z:

None

Term 2025/26Z:

None

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 2600-S1-CM-MTD in USOSweb