In vitro cell culture as a tool in biomedicine 2600-S1-CM-HKV

The lectures are an introduction to the issues related to the cytophysiology of cells in in vitro culture. Lecture topics:
1 / Introduction to in vitro cell culture, culture environment
2 / Cytophysiology of animal and human cells
3 / Cell cultures as a research model

Laboratory classes will make it possible to learn the principles of in vitro animal cell culture and their practical use as a tool in biomedicine. Exercise topics:
1/ Principles of in vitro animal cell culture, work under aseptic conditions, experiment planning.
2/ Establishing a primary culture of normal cells using the explant and enzymatic methods.
3/ Secondary cultures - passaging of different cell types. Methods of freezing and storage of animal cells.
4/ Analysis of the cytotoxic compounds influence on cultured cells. Assessment of cell viability - trypan blue staining, MTT test.
5/ Seminar "Application of in vitro culture in diagnostics and therapy". Final test.

Total student workload

Contact hours (27 h): lectures - 5 h, laboratory classes - 20 h, consultations with teacher - 2 h Student's individual work (23 h): - preparation to laboratory classes – 5 h - preparing presentation, writing reports – 8 h - preparation to pass the final test – 10 h Total time = 50 h = 2 ECTS

Learning outcomes - knowledge

W1: student has the knowledge of animal cells cytophysiology, metabolic processes, the cell cycle, regulation of cell proliferation and death - K_W03, K_W06 W2: knows the methodology for establishing and maintaining animal cells in in vitro culture - K_W04 W3: understands the necessity to consider the rules of aseptic working, knows the methods of handling biological material and disposal of biological samples - K_W06, K_W09 W4: knows the ways of using in vitro cell culture as a model in basic research, cytotoxicity, tissue and organ regeneration - K_W08, K_W10, K_W11

Learning outcomes - skills

U1: student has the ability to work under aseptic conditions, prepare sterile reagents and media for in vitro culture, and establish the cell culture - K_U04, K_U10 U2: can maintain the animal cells in in vitro culture, passage and freeze cells properly - K_U01, K_U05 U3: can assess the viability and metabolism of cells in culture as well as the influence of toxic substances - K_U03, K_U05 U4: can plan perform experiments and interpret the obtained results - K_U04, K_U08 U5: student has the ability to read and understand professional literature in Polish and English and is able to critically evaluate the information obtained - K_U09, K_U11

Learning outcomes - social competencies

K1: student is responsible for the equipment and team working on the implementation of tasks - K_K02 K2: is aware of the importance of predicting the effects of working with biological material, and maintains safety rules - K_K05 K3: understands the need for continuous improvement of professional competences - K_K01

Teaching methods

Lecture: informative lecture with multimedia presentations Laboratory classes: students have to perform the experiments according to the obtained protocol under aseptic conditions. Other teaching methods used are: presentations, discussion, reports. The experiments have to be conducted under aseptic conditions therefore lab groups should be as small as possible (max. 8-10 persons).

Expository teaching methods

- participatory lecture
- informative (conventional) lecture

Exploratory teaching methods

- experimental
- laboratory
- seminar
- observation
- presentation of a paper
- practical

Type of course

compulsory course

Prerequisites

Basic knowledge in biochemistry and cell biology

Course coordinators

Katarzyna Roszek

Assessment criteria

Lectures:
- written test consisting of single-choice questions: K_W03, K_W06, K_W08, K_W10, K_W11

Classes:
- attendance at laboratory classes
- evaluation of the current preparation and activity during classes: K_K01, K_K02 (30% of the final grade)
- evaluation of the multimedia presentation prepared by the student on a specific topic: K_U09, K_U11 (20%)
- written final test: K_W03, K_W04, K_W06, K_W11 (50%)

Assessment criteria: required threshold for a satisfactory assessment (3.0) - 55-60%, satisfactory plus (3.5) - 61-70%, good (4.0) - 71-80%, good plus (4.5) 81-90%, very good (5.0) - 91-100%

Practical placement

not applicable

Bibliography

1. Stokłosowa S. „Hodowla komórek i tkanek” (PWN, Warszawa 2012, 2017)
2. Kilarski W. „Strukturalne podstawy biologii komórki” (Wydawnictwo Naukowe PWN, Warszawa 2003)
3. Kawiak J. „Seminaria z cytofizjologii dla studentów medycyny, weterynarii i biologii” (Elsevier Urban & Partner, Wrocław 2002)
4. Kłyszejko-Stefanowicz L. „Cytobiochemia” (Wydawnictwo Naukowe PWN, Warszawa 2002)

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 2600-S1-CM-HKV in USOSweb