Enzymes in biomedicine 2600-S1-CM-EBIO

Course content: structure, functions of enzymes, modifications of enzymatic activities, and the possibility of using the above information in biomedicine:
1. Structure and properties of enzymes and mechanisms of regulating their activity
2. The role of enzymes in signaling at the cellular level; damage to biological signal transmission processes
3. Participation of enzymes in selected physiological processes, etiology of diseases and diagnostics: integration of metabolism, intra-organ regulation and disorders of protein, sugar, and fat metabolism at the level of organs, metabolic disorders, and blocks, and other enzymopathies
4. The use of enzymes as diagnostic markers
5. The use of enzymes as diagnostic tools and biotechnological tools used in biomedicine.

Laboratory classes: learning and practical use of knowledge about catalytically active proteins, mechanisms of biochemical changes in the course and diagnosis of diseases, and modern diagnostic tests, constructed based on enzymes:
1. Kinetics of marker enzymes, pathobiochemistry, diagnosis and molecular therapy of enzymatic storage diseases, characteristics of enzymes related to the etiology of lysosomal diseases
2. The use of enzymes to construct diagnostic tests:
- enzymes as markers: Quantitative and qualitative analysis of molecular markers of ischemic states
- enzymes as diagnostic tools: the use of enzyme tests for the determination of lipid compounds and blood glucose
3. Applications of rapid, enzymatic diagnostic tests for the quantitative analysis of metabolites and pathogens

Total student workload

Since academic year 2024/25: 1. Hours conducted with the participation of instructors: - contact hours provided in the study plan for a given course: 25h (10h – lectures, 15h lab classes) - the number of “in-class” contact hours as well as consultation hours: 8h 2. The time devoted to self-study of a student: 7h 3. The time required to prepare for assessment and grading: 10h

Learning outcomes - knowledge

W1: The student has advanced and up-to-date knowledge of the structure of enzymes and the regulation of their activity: K_W01, K_W03, K_W06, K_W08, W2: The student has advanced and up-to-date knowledge of the participation of enzymes in selected physiological processes; enzyme dysfunction in the etiology of diseases and the use of enzymes in diagnostics: K_W01, K_W03, K_W06, K_W08, W3: The student lists and describes the methods of enzyme production and enzymatic methods used in the diagnosis of physiological and metabolic disorders and diseases; knows the sensitivity, specificity, and limitations of selected enzymatic methods: K_W02, K_W04

Learning outcomes - skills

U1: The student can describe the methods of enzyme regulation and the influence of activity deficiencies on the biochemical processes of organisms K_U01, K_U03, K_U08 U2: The student can use the knowledge to indicate the relationship between the properties of enzymes and the possibility of using them as disease markers or tools for constructing diagnostic tests K_U01, K_U03, K_U08 U3: The student can interpret the obtained results and critically evaluate classic and modern diagnostic tests K_U03, K_U05, K_U10 U4: The student uses advanced measurement and analytical techniques as well as qualitative and quantitative methods for the diagnosis of biological material: K_U04, K_U05

Learning outcomes - social competencies

K1: The student understands the need to regularly update knowledge due to the dynamic development taking place in the field of medical biochemistry and diagnostics: K_K01 K2: The student can rationally and critically select information obtained from scientific literature, the Internet, and other mass media sources, regarding the sensitivity of research methods or the basis of diseases K_K08, K_K09, K_K10

Teaching methods

Lecture: information lecture with multimedia presentations Laboratory classes: demonstration, exercises, experiments, laboratory method Online learning if necessary: methods for the presentation of content, methods relating to real or fictional situations, methods based on cooperation, methods for developing reflective thinking, methods for exchange and discussion Students work with the protocol in teams of 2-3 people (the class should have a maximum of 12 people per lecturer), taking into account the methodology of experiments and observations.

Expository teaching methods

- informative (conventional) lecture
- participatory lecture

Exploratory teaching methods

- experimental
- practical
- observation
- laboratory

Online teaching methods

- cooperation-based methods

Prerequisites

Basic knowledge of biochemistry and genetics.

Course coordinators

Joanna Czarnecka

Assessment criteria

Lectures: test containing single-choice closed questions and open-ended questions. Passing the test after reaching at least 50% of the available points. A very good grade requires the achievement of more than 90% of the available points. Other degrees are set proportionally in the range of 50-90%
W01, W02, W04, W06, W08

Laboratory: the following points are fulfilled:
1 / class attendance K01
2 / Passing the reports of the experiments: W02, W04, W010, U05
3 / Written test (70% of the final grade): W01, W04, W06, W08, W11, U3, U5, U8
Passing the test after reaching at least 50% of the available points. A very good grade requires the achievement of more than 90% of the available points. Other degrees are set proportionally in the range of 50-90%
4 / student activity, current preparation for classes (30% of the final grade) W01, W05, W06, U01, U04, U05, U08, K03

Practical placement

Bibliography

- Harper Biochemistry, R.K. Murray, D.K. Granner, V.W.Rodwell
- Biochemistry, J.M Berg, J.L.Tymoczko, L.Strye
- Molecular biology of the cell, Alberts B et al.
- Color Atlas of Biochemistry, Koolman J, Röhm K-H
Recommended:
- Actual papers in the biochemistry field

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 2600-S1-CM-EBIO in USOSweb