Cell biology 2600-S1-CM-BK

The lecture present the structural and functional organization of the cell as a basic system of biological activity, which is a highly complex and perfectly integrated unit of structure, function and reproduction. Content of lecture:
• Spatial and functional organization of the nucleus. Chromatin, chromosomes, mutations and genetic diseases. Mechanisms of regulation of gene expression (transcription factors, epigenetics).
• Intracellular compartments, protein sorting and transport - organization and functions of the endoplasmic reticulum and the Golgi apparatus.
• Vesicular transport, endocytosis and exocytosis, intracellular digestion.
• Organization and functions of the cell membrane - transmembrane transport and cell signaling. Mechanisms of drugs transport across biological membranes.
• Cell cycle control. Cell senescence and death.
• Mitosis and meiosis.

During the laboratory classes student independently performs microscopic preparations and uses the known techniques of cell biology, conducts experiments and microscopic observations. Student documents and interprets the obtained results using terminology and the acquired knowledge. Solving the research problems student explains the relationships between the structure and function of individual compartments of the eukaryotic cell and describes the basic cytophysiological processes.

Lectures:
1. Spatial and functional organization of the nucleus.
2. Mechanisms of regulation of gene expression.
3. Cell cycle control. Cellular senescence and death.
4. Intracellular compartments. Protein sorting and transport, intracellular digestion.
5. Structure and functions of the plasma membrane. Mechanisms of drug transport across biological membrane.
Laboratory classes:
1. Identification of intracellular compartments at light microscopy level. Observation and staining of living cells.
2. Ultrastructural analysis of cell – electron microscopy.
3. Nucleic acids. The pattern of chromatin organization in the interphase nucleus. DNA replication.
4. Bioimaging techniques of gene expression.
5. Chromatin distribution to daughter cells during mitosis.
6. Reduction of genetic material during meiosis. Elements of cytogenetics.

Term 2021/22Z:

None

Term 2022/23Z:

None

Term 2023/24Z:

None

Term 2024/25Z:

None

Total student workload

Contact hours (40 h): - lectures - 10 h - laboratory classes – 20 h - consultations with teacher - 10 h Student's individual work (35 h): - preparation to laboratory classes – 10 h - preparation to pass the final lab test - 10 h - preparation to pass the final test – 15 h Total number of hours 75 h

Learning outcomes - knowledge

W1: identifies and describes the structure of subcellular organelles and explains the basic cellular life processes – K_W03, K_W11 W2: explains the basic issues of plant and animal cell biology used in research conducted within the studied field – K_W03, K_W11 W3: explains the relationship between cell dysfunction and disease (pathological) conditions in the human organism – K_W03, K_W08, K_W11 W4: knows techniques used in the study of cell biology, such as light and electron microscopy, cytochemical staining, immunocytochemistry, in situ hybridization – K_W03, K_W04, K_W11

Learning outcomes - skills

U1: applies the knowledge and learned techniques in the practical solving of research problem in the field of cell biology – K_U01, K_U02, K_U04, K_U05, K_U10 U2: analyzes and interprets microscopic images at the cellular and ultrastructural level – K_U03, K_U04, K_U05 U3: uses available sources of scientific information to verify and correctly interpretation obtained results – K_U03, K_U08, K_U09, K_U11

Learning outcomes - social competencies

K1: is aware of the progress in knowledge in the field of cell biology and understands the need to constantly expand it and improve his professional competences – K_K01, K_K04, K_K06 K2: can work in a team and coordinate the work of research group, strives to perform entrusted tasks – K_K02, K_K03 K3: follows the rules of ethics and understands the social aspects of the practical use of knowledge and skills within the field of study – K_K05, K_K06

Teaching methods

Lecture: informative lecture with multimedia presentation Laboratory: theoretical introduction – multimedia presentation, discussion, practical part – students (2-3 persons in a team) have to perform the tasks according to the obtained protocol, supervised by the person conducting the classes

Observation/demonstration teaching methods

- display

Expository teaching methods

- problem-based lecture
- informative (conventional) lecture

Exploratory teaching methods

- experimental
- laboratory
- observation
- practical

Type of course

compulsory course

Prerequisites

Course coordinators

Katarzyna Niedojadło

Assessment criteria

Lecture: a final test consisting of single-choice and multiple-choice questions – K_W03, K_W04, K_W08, K_W11, K_U01, K_U02, K_U04, K_U05, K_U10, K_K01, K_K04, K_K06

Laboratory classes: a final test consisting of single-choice and multiple-choice questions – K_W03, K_W04, K_W08, K_W11, K_U01, K_U02, K_U04, K_U05, K_U10, K_K01, K_K04, K_K06
Activity – K_K01, K_K02, K_K03

Assessment criteria: 100-91% very good (5,0), 81-90% good plus (4,5), 71-80% good (4,0), 61-70% satisfactory plus (3,5), 55-60% satisfactory (3,0), 54-0% unsatisfactory (2,0)

Practical placement

Bibliography

1. Alberts B., D. Bray, K. Hopkin, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter. Postawy biologii komórki. Tom 1 i 2 + CD. Wyd. II. Wydawnictwo Naukowe PWN, 2009
2. Brown T.A. Genomy. Wydawnictwo Naukowe PWN, 2009
3. Allison LA. Podstawy Biologii Molekularnej. Wydawnictwo Uniwersytetu Warszawskiego, 2011
4. Turner P, McLennan A, Bates A, White M. Krótkie wykłady z biologii molekularnej. Wydawnictwo Naukowe PWN, 2012
5. Kawiak J., J. Marecka, M. Olszewska, J. Warchoł. Podstawy cytofizjologii. Wydawnictwo Naukowe PWN, 1997
6. Kilarski W. Strukturalne podstawy biologii komórki. Wydawnictwo Naukowe PWN, 2003
7. Litwin J. A., M. Gajda. Podstawy technik mikroskopowych. Wydawnictwo Uniwersytetu Jagiellońskiego, 2011
8. Kurczyńska E., D. Borowska-Wykręt. Mikroskopia świetlna w badaniach komórki roślinnej. Ćwiczenia. Wydawnictwo Naukowe PWN, 2007
9. Wróbel B., K. Zienkiewicz, D. J. Smoliński, J. Niedojadło,
M. Świdziński. Podstawy mikroskopii elektronowej. Wydawnictwo Uniwersytetu Mikołaja Kopernika, 2005

Term 2021/22Z:

None

Term 2022/23Z:

None

Term 2023/24Z:

None

Term 2024/25Z:

None

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 2600-S1-CM-BK in USOSweb