Conducted in terms: 2021/22Z, 2022/23Z, 2023/24Z, 2024/25Z

ISCED code: 0512

ECTS credits: 3

Language: Polish

Organized by: Faculty of Biological and Veterinary Sciences

Biochemistry 2600-S1-CM-BCH

http://- (term 2021/22Z)
http://- (term 2022/23Z)
http://- (term 2023/24Z)
http://- (term 2024/25Z)

Students gain theoretical and practical knowledge about the structure and function of biomolecules and metabolic processes in which energy is transformed into a biologically useful form.
Lecture: Amino acids and proteins: structure, properties, and functions (special attention to the relationship between structure and function). Sugars: classification, nomenclature, isomerism, monosaccharides, disaccharides, polysaccharides, glycosides - glycosidic bonds, glycoproteins. Nucleic acids: structure, replication, and transcription processes; protein biosynthesis. Lipids: Classification, structure, and functions. Biological membranes: structure, functions, and transport across membranes. Enzymes: structure, classification, nomenclature, coenzymes, the kinetics of enzymatic reactions. Basic concepts of metabolism: energy sources, the general strategy of metabolic transformation of sugars, proteins, and fats, ATP as an energy carrier, high-energy bonds, and types of phosphorylation. Obtaining energy in metabolic processes: glycolysis, oxidative decarboxylation of pyruvate, Krebs cycle. The respiratory chain as the last step in cellular oxidation. Energy balance of glucose oxidation.

Laboratory exercises are aimed at introducing the student to various laboratory techniques enabling quantitative and qualitative analysis of the chemical components of an animal cell. The following topics will be covered during the exercises: Biochemical calculations. Amino acids - structure and properties. Determination of protein concentration by the biuret method. Serum protein electrophoresis. Preparation and analysis of DNA from nuclei of isolated and etiolated wheat seedlings. Characteristic reactions of sugars. Kinetics of enzymatic reactions.

Term 2021/22Z:

As above (in A. General description of the course)

Term 2022/23Z:

As above (in A. General description of the course)

Term 2023/24Z:

As above (in A. General description of the course)

Term 2024/25Z:

As above (in A. General description of the course)

Total student workload

1. Hours conducted with the participation of instructors: - contact hours provided in the study plan for a given course: 45h (15h – lectures, 30h lab classes) - the number of “in-class” contact hours as well as consultation hours: 10h 2. The time devoted to self-study of a student:20h 3. The time required to prepare for assessment and grading: 10h

Learning outcomes - knowledge

W1: The student knows the chemical structure, properties, and functions of proteins, nucleic acids, lipids, and saccharides: K_W01, K_W05 W2: The student knows the relationship between the structure of proteins, nucleic acids, lipids, and saccharides and their function in organisms K_W06, K_W08, K_W11. W3: The student defines and explains catabolic and anabolic processes in the cell, and characterizes the processes of processing, storing, and releasing energy: K_W01, K_W08, K_W11. W4: The student knows the rules of preparing reports, studies, diploma theses, and publications: K_W02, K_W04, K_W010 W6: The student lists and defines chemical reactions allowing for the detection of biomolecules, methods of separation and quantification of the concentration of biomolecules, and analysis of enzyme activity; knows the theoretical and practical aspects of spectrophotometric methods, chromatography, electrophoresis): K_W02, K_W04, K_W05 W7: The student knows the basic principles of occupational health and safety during laboratory classes (K_W09).

Learning outcomes - skills

U1: The student is able to locate and describe the processes related to the energy conversion in the cell and the regulation of metabolic pathways: K_U01, K_U03, K_U07, K_U08 U2: The student can explain the issues related to the course of biochemical transformations of saccharides, proteins, fats, and nucleic acids (K_U01, K_U03, K_U07, K_U08). U3: The student is able to carry out quantitative and qualitative analyzes of biomolecules in biological material: K_U01, K_U04, K_U05 U4: The student can prepares documentation, interprets the results of the experiments and correctly draws conclusions: K_U01, K_U08, K_U09 U5: The student understands and assesses the risks associated with working with biological material: K_U10

Learning outcomes - social competencies

K1: Understands the need to deepen knowledge in the field of biochemistry: (K_K01) K2: The student critically relates to the current opinions and analyzes them based on the acquired knowledge (K_K01, K_K06). K3: The student can organize work, and is responsible for their safety and the safety of others (K_K02, K_K05).

Teaching methods

Lecture: information lecture with multimedia presentations Laboratory classes: demonstration, exercises, experiments, laboratory method Online learning if necessary: methods for the presentation of content, methods relating to real or fictional situations, methods based on cooperation, methods for developing reflective thinking, methods for exchange and discussion Students work in teams of 2-3 people (the class should have a maximum of 12 people per lecturer), taking into account the methodology of experiments and observations. They will conduct experiments following the protocol and after discussing the theoretical foundations and planning the work as well as safety rules.

Observation/demonstration teaching methods

- display

Expository teaching methods

- informative (conventional) lecture
- narration
- participatory lecture

Exploratory teaching methods

- experimental
- practical
- observation
- laboratory

Online teaching methods

- exchange and discussion methods
- methods referring to authentic or fictitious situations
- methods developing reflexive thinking
- games and simulations
- content-presentation-oriented methods
- cooperation-based methods

Type of course

compulsory course

Prerequisites

The student knows the structure of the plant, animal, and bacterial cells, and lists and characterizes cell organelles.

Course coordinators

Joanna Czarnecka

Assessment criteria

Lectures:
Written exam, test containing single-choice closed questions and open-ended questions. Passing the exam after reaching at least 50% of the available points. A very good grade requires the achievement of more than 90% of the available points. Other degrees are set proportionally in the range of 50-90%
W01, W05, W04, W06, W08, U01, U02

Laboratory: the following points are fulfilled
1 / class attendance K01
2 / Passing the reports from experiments: W02, W04, W010, U05
3 / Written test (70% of the final grade): W01, W04, W05, W06, W08, W11, U2, U3, U4, U5
Passing the test after reaching at least 50% of the available points. A very good grade requires the achievement of more than 90% of the available points. Other degrees are set proportionally in the range of 50-90%

4 / student activity, current preparation for classes (30% of the final grade) W01, W05, W06, U01, U04, U05, U08, K03

Practical placement

Bibliography

- Harper Biochemistry, R.K. Murray, D.K. Granner, V.W.Rodwell
- Biochemistry, J.M Berg, J.L.Tymoczko, L.Strye
- Molecular biology of the cell, Alberts B et all.
- Color Atlas of Biochemistry, Koolman J, Röhm K-H
Recommended:
- Actual papers in the biochemistry field

Term 2021/22Z:

As above (in A. General description of the course)

Term 2022/23Z:

As above (in A. General description of the course)

Term 2023/24Z:

As above (in A. General description of the course)

Term 2024/25Z:

As above (in A. General description of the course)

Notes

Term 2021/22Z:

Term 2022/23Z:

Term 2023/24Z:

Term 2024/25Z:

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 2600-S1-CM-BCH in USOSweb