Molecular Biology 2600-OG-EN-MB

1.DNA isolation
2 PCR amplification of isolated genomic DNA using universal primers
3.Sequencing

Total student workload

Contact hours with teacher: - participation in laboratory 30 hrs - consultations- 15 hrs Self-study hours: - preparation for laboratory 20 hrs - reading literature- 10 hrs Altogether: 75 hrs (3 ECTS)

Learning outcomes - knowledge

Student W1: has advanced knowledge of basic process of isolation of DNA from various sources: blood, tissue etc.; W2: knows that different types of DNA require different methods of isolation; W3: is familiar with basic knowledge about DNA structure W4: is acquainted with agarose gel electrophoresis can be used to separate DNA fragments of different sizes;

Learning outcomes - skills

Student U1: is able to/ has the ability to perform all process steps from DNA isolation to sequencing U2: is capable of efficiently use the basic equipment used in the molecular laboratory U3: has advanced skills in PCR and electrophoresis methods U4: can explain prosesses of isolation DNA, PCR methods, cleaning DNA, sequencing

Learning outcomes - social competencies

Student K1: understands the significance of independent work K2: understands the need for precision and reliability of tasks performed K3: is aware of the hazards in the molecular laboratory K4: can cooperate/ communicate/ work with another students

Course coordinators

Term 2024/25Z:

Milena Kulasek

Term 2025/26Z:

Natalia Mucha
Jarosław Tyburski

Term 2023/24Z:

Paulina Trzeciak

Term 2022/23Z:

Paulina Trzeciak

Teaching methods

Expository teaching methods: - informative lecture - laboratory

Type of course

elective course

Learning outcomes

Student
W1: has advanced knowledge of basic process of isolation of DNA from various sources: blood, tissue etc.;
W2: knows that different types of DNA require different methods of
isolation;
W3: is familiar with basic knowledge about DNA structure
W4: is acquainted with agarose gel electrophoresis can be used to separate DNA fragments of different sizes;

Assessment criteria

Assessment methods:
- activity, attendance
Assessment criteria: attendance
The beginning of class may also include handing in
assignments and discussion among the class that will be scored
Students should inform the instructor of the reason for their absence as soon as possible – ideally, before the class is missed.

fail- less than 50 %
satisfactory- 51 %
good – 70 % attendance + activity
good plus- 90 % attendance + activity
very good- 100 % attendance + activity

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 2600-OG-EN-MB in USOSweb