Regenerative Medicine
1655-Lek5MREGE-J
Lectures on the subject of Regenerative Medicine will present the definitions of tissue engineering and regenerative medicine. During the classes, the phylogenetic relationships between healing and regeneration, as well as the principles of induced regeneration, will be explained. Concepts of tissue regeneration using Advanced Therapy Medicinal Products (ATMP) will be discussed, including: somatic cell therapy medicinal products and tissue engineering products. Regenerative medicine is a constantly developing field of science, therefore one of the problems discussed during the lectures will be the dangers resulting from cell and tissue transplantation and the causes of failure in the transplantation of cultured cells and tissues. An important element of the regeneration of organs and tissues in vitro is the use of stem cells, therefore the types of stem cells will be discussed, including naturally occurring cells and induced cells. The use of some types of stem cells is controversial, therefore ethical aspects regarding stem cells in biology and medicine, as well as topics regarding therapeutic cloning, will be presented. Creating a product using tissue engineering methods for use in clinical practice requires the use of Good Manufacturing Practice (GMP), therefore the regulations and requirements that must be met in order for cells, neo-tissue, neo-organ produced in the laboratory to be implanted to the patient will be presented.
The tutorials will be divided into four thematic blocks: regenerative medicine in plastic surgery, urology, orthopedy, and dermatology. Students will become familiar with the practical aspects of work in a tissue engineering laboratory, learn the method of isolating mesenchymal stromal cells from adipose tissue, the method of their cultivation, passage, and their practical use in urology, dermatology, orthopedy, and plastic surgery. Methods of decellularization/recellularization of organs and tissues and their potential application in regenerative medicine will be presented. During practical classes, implants will be prepared for the regeneration of organs such as the urinary bladder, urethra, and ureters. Students will be able to see how the form of cell administration can influence the therapeutic effect. Dermatology is a field of medicine that was one of the first to use regenerative medicine techniques in clinical practice. During the classes, students will be presented with types of skin grafts grown in vitro, methods of their preparation, and methods of applying these constructs to wounds.
Total student workload
1.Workload associated with direct participation of academic teachers:
- lectures: 8 h
- tutorials: 10 h
- final test: 1 ,5 h
Total workload involving the direct participation of academic teachers: 19,5 h, which equals 0,78 of an ECTS point.
2. Total student workload:
- lectures: 8 h
- tutorials 10 h
- preparation for tutorials (including reading of the selected literature and written tasks completion): 5 h
- preparation for final test and final test: 3 + 1,5 = 4,5 h
Total student workload: 27,5 h, which equals 1.1 ECTS point.
3. Workload related to conducted scientific research:
- reading the indicated scientific literature: 5 h
- participation in lectures (taking into account the results of research and scientific literature in the field of regenerative medicine): 4 h
- participation in tutorials (taking into account the results of research and scientific literature in the field of regenerative medicine): 5 h
- preparation for final test (taking into account scientific studies in the field of regenerative medicine): 3h
Total student workload: 17 h, which equals 0.68 ECTS point.
4. Time required to prepare and participate in the assessment process:
- preparation for final test and final test: 3 + 1,5 = 4,5 h
Total student workload: 4h, which equals 0.18 ECTS point.
5. Total student practical workload:
- tutorials: 10 h
Total student practical workload: 10h, which equals 0.4 ECTS point.
6. Time required to complete the mandatory internship:
Not applicable
Learning outcomes - knowledge
W1: Discusses basic issues in the field of biology and stem cell functions. Knows the role of stem cells in the body's aging processes, cancerogenesis, healing, and regeneration (B.W19)
W2: Characterizes the sources of stem cells (embryonic, fetal and adult origin), techniques of induced pluripotent stem generation and methods of transforming them into differentiated cells (A.W5); (B.W19); (C.W42).
W3. Lists basic cell culture techniques including isolation and establishment of primary culture, cell multiplication in static and dynamic culture conditions (bioreactor) (B.W29); (C.W42).
W4: Lists methods of producing neo-tissues and neo-organs using tissue engineering techniques, including decellularization, tissue recellularization, and bioprinting (B.W29); (C.W42).
W5: Has knowledge about the production of Advance Therapy Medicinal Products (ATMP) including: somatic therapy medicinal products cellular and tissue engineered products, in accordance with Good Manufacturing Practice guidelines (B.W26); (C.W42).
W6: Lists ways to use ATMP products in treatment of selected diseases (B.W26); (C.W42).
W7: Describes the principles of conducting in vitro and in vivo experimental research in the field of tissue engineering and regenerative medicine for the development of medicine (B.W29); (C.W42).
Learning outcomes - skills
U1: Is able to isolate and establish a primary culture of mesenchymal stromal stem cells (B.U13).
U2: Can prepare a neo-organ or neo-tissue for clinical applications using tissue engineering techniques (B.U13).
U3: Is able to adapt the type of implant produced using tissue engineering methods to therapeutic requirements (B.U13).
U4: Uses clinical research databases (B.U11).
U5: Recognizes own limitations, self-assesses deficits and educational needs in the field of regenerative medicine (B.U11).
U6: Critically analyzes medical literature and draws conclusions
based on the literature (B.U11)
Learning outcomes - social competencies
K1: Is aware of his own limitations and the need for constant education (K_K07).
K2: Is aware of the need to cooperate with representatives of other professions in the field of health care, especially in the field of medical biotechnology (K_K06).
Teaching methods
Lectures:
• informational lecture
• conversational lecture
• educational discussion
• analysis of case reports
Tutorials:
• laboratory exercises
• educational discussion
• analysis of case reports
• design and analysis of scientific research
Observation/demonstration teaching methods
- display
Type of course
compulsory course
Prerequisites
Students beginning their first aid education should possess a basic understanding (high school level) of histology.
Course coordinators
Assessment criteria
Written test (0-10 pkt.): W3-W7
Task realization: report preparation (0-10 pkt.): W3-W7; U1-U6
Practical test (0-10 pkt.): U1-U6
Passing based on the appropriate number of points (0-30 pkt., >60%):
Final written test (>60%): W1-W7; U1-U6.
Extended observation ( > 50%): K1 – K2
In order to be eligible to take the final written test, the student is required to pass the oral and practical tests. A positive social competence assessment of the student is also required.
Practical placement
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: