Cell Culture Models in Preclinical Studies
1655-Lek21MOKO-J
The seminars aim at describing cellular models of human diseases in pre-clinical research. Students will learn how three-dimensional cell cultures mimic the complex tissue microenvironment allowing for analyzing cell-cell interactions, preserving signaling activity and gene expression. The tutorials are partially thematically related to the topics discussed at the seminars. Students will learn about the basic principles of in vitro cell culture and the sources of cells for pre-clinical research. They will analyze the influence of biologically active compounds on the properties of cultured cells, including their morphology and metabolic activity. Finally, they will establish a three-dimensional cell culture.
Total student workload
Study hours involving teacher participation:
- seminars – 6 hours
- tutorials – 6 hours
- examination – 1 hour
Total workload involving the direct participation of academic teachers: 13 hours, which equals 0.52 of an ECTS point.
Study hours involving individual student work:
- seminars – 6 hours
- tutorials – 6 hours
- preparation for tutorials – 9 hours
- preparation for examination and exam – 9 hours
Total student workload: 30 hours, which equals 1,2 ECTS point.
Workload associated with research:
- reading of scientific literature: 1,5 hours
- participation in seminars (related to research in the field of pre-clinical cell culture models): 4 hours
- participation in tutorials (related to research in the field of pre-clinical cell culture models): 6 hours
- final test preparation (related to research in the field of pre-clinical cell culture models): 2,5 hours
Total student workload associated with research: 14 hours, which equals 0.56 ECTS point.
Time requirements for preparation and participation in the student assessment process:
- preparation for final test: 8 + 1 = 9 hours (0.36 of an ECTS point).
Student workload connected to distant learning methods: not applicable
Student workload connected to practical skills classes:
- participation in tutorials: 6 hours (0.24 of an ECTS point)
Time required to complete the obligatory internship: N/A
Learning outcomes - knowledge
W1: Justifies the use of the extracellular matrix as a scaffold for 3D cultures (A.W5)
W2: Lists modifications of the extracellular matrix enabling directed differentiation of cultured cells (A.W5)
W3: Explains the use of stem cells in establishing 3D cultures (B.W19)
W4: Compares the properties of stem cells grown in monolayers and 3D cultures (B.W19)
W5: Defines the possibilities of using cell cultures in modeling human diseases (C.W42)
Learning outcomes - skills
U1: Prepares essential reagents for in vitro cell culture (B.U5)
U2: Analyzes the biological properties of cells using spectrophotometric techniques (B.U8)
U3: Plans and conducts experiments with the use of cell cultures (B.U13)
U4: Analyzes the results of conducted experiments and draws correct conclusions (B.U13)
Learning outcomes - social competencies
K1: Is willing to deepen knowledge and improve own skills (K.K1)
K2: Shows interest in updating his knowledge (K.K8)
Teaching methods
Seminars:
- problem lecture
- seminar lecture
- didactic discussion
Tutorials:
- laboratory exercises
- research design and analysis
Type of course
compulsory course
Prerequisites
Elementary knowledge of cell biology.
Course coordinators
Practical placement
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: