Alternative Preclinical Models with IT Analysis
1655-Lek21AMPI-J
This course covers the fundamentals of cell culture in healthcare through a combination of seminars and hands-on exercises. In the seminars, students will explore cellular models of human diseases, which offer alternatives to using laboratory animals in preclinical research. They'll also delve into the creation of three-dimensional cell cultures that mimic complex tissue environments, enabling the study of cell interactions and the maintenance of signaling pathways and gene expression in vitro.
The exercises complement the seminar topics. Students will gain practical experience in both two-dimensional and three-dimensional cell culture techniques, as well as learn about cell sources for preclinical research. Additionally, they'll be introduced to key clinical data repositories and learn how to navigate them effectively. During the practical sessions, students will experiment with biologically active compounds to observe their effects on various aspects of cell growth, such as morphology and metabolic activity. They'll then collect and analyze the data generated, working towards creating and interpreting a comprehensive database of their findings.
Total student workload
1.Workload associated with direct participation of academic teachers:
- seminars: 6 h
- tutorials: 12 h
- final test: 1 h
Total workload involving the direct participation of academic teachers: 19 h, which equals 0.76 of an ECTS point.
2.Total student workload:
- seminars: 6 h
- tutorials: 12 h
- preparation for tutorials (including reading of the selected literature and written tasks completion): 9 h
- preparation for final test and final test: 9.5 + 1 = 10.5 h
Total student workload: 37.5 h, which equals 1.5 ECTS point.
3. Workload related to achievement of learning outcomes in medical simulation settings:
Not applicable
4. Workload associated with achievement of learning outcomes related to medical communication:
Not applicable
Learning outcomes - knowledge
W1: Justifies the use of extracellular matrix as a scaffold for cell culture in 3D (A.W3)
W2: Lists modifications of the extracellular matrix enabling directed cell differentiation (A.W3)
W3: Explains the use of stem cells in establishing spatial cultures (B.W18)
W4: Compares the properties of stem cells cultured in a monolayer and in 3D cultures (B.W18)
W5: Determines the possibilities of using cell cultures in modeling human diseases (C.W33)
W6: Discusses the basic concepts of information system analysis, as well as the role of information resources (B.W23)
W7: Lists and characterizes the basic methods, techniques and tools in the field of analysis, design and construction of IT systems (B.W23)
W8: Discusses the types of commercial and medical databases (BW.23)
Learning outcomes - skills
U1: Is able to prepare basic reagents for establishing and conducting in vitro cell cultures (B.U5)
U2: Plans and carries out experiments using cell cultures (B.U11)
U3: Analyzes the biological properties of cells using spectrophotometric techniques (B.U11)
U4: Analyzes the results of experiments and draws correct conclusions based on them (B.U11)
U5: Is able to interpret and prepare documentation in the form of an IT task (B.U8)
U6: Is able to assess the usefulness of IT methods and tools for problems related to databases in the context of preclinical, clinical and personal research (B.U8)
Learning outcomes - social competencies
K1: Demonstrates willingness to deepen knowledge and improve own skills (K_K05)
K2: Shows interest in updating knowledge (K_K08)
K3: Demonstrates an attitude of self-education (K_K05)
K4: Demonstrates an attitude of cooperation in a group (K_K09)
Teaching methods
Seminars:
• problem lecture
• conversation lecture
• didactic discussion
Tutorials:
• laboratory exercises
• design and analysis of scientific research
• demonstration with instructions
• subject exercises
• work with computer
Computer-assisted learning:
• medical databases
Prerequisites
Students beginning their education on alternative preclinical models with informatics analysis should have a foundational understanding of cell biology, informatics and computer usage (high school level).
Course coordinators
Assessment criteria
Reports (≥60%): U1-U6
Final written test (≥60%): W1-W8, U4, U6
Extended observation (> 50%): K1-K4
To be eligible to take the final written test, students must achieve an
overall score of at least 60% from tutorial reports. Additionally, a positive assessment of social competence is required.
Practical placement
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: