Course Selection: Tissue Engineering
1655-Lek12WYBINZ-J
During the first class in the cell culture laboratory, the plan and equipment of the laboratory will be presented, along with the rules of safety and asepsis. Students will learn about the basic reagents used for cell culture, such as media and supplements. The aim of the exercises is to get the knowledge about the basic techniques used in a tissue engineering laboratory, such as: cell passaging, counting the cells using a hemocytometer, changing the culture medium. Students will learn the technique of rat femoral and tibial collecting in order to isolate bone marrow stromal cells and pancreatic collecting in order to isolate rat pancreatic islets. By completing the course, students will be able to collect biological material from laboratory animals, establish the primary cell culture, isolate pancreatic islets, and conduct three-dimensional cell culture.
Total student workload
1. Study hours involving teacher participation:
- tutorial – 10 hours
- final test – 3 hour;
Study hours involving teacher participation is 13 hours which corresponds to 0,52 ECTS
2. Study hours involving individual student work:
- participation in tutorial – 10 hours
- preparation for tutorials (including reading the indicated
literature ) – 7 hours;
- preparation for final test and final test – 5 + 3 = 8 hours;
Study hours involving individual student work is 25 hours which corresponds to 1 ECTS
3. Study hours involving scientific research:
- reading scientific literature – 7 hours;
- participation in tutorial (including research results and
scientific reports from the field of tissue engineering) – 5
hours
- preparation for final test (including scientific reports for the field of tissue engineering) – 7 hours
Study hours involving scientific research is 19 hours which corresponds to 0,76 ECTS
4. Time required to prepare and to participate in the assessment process:
- preparation for final test and final test – 5 + 3 = 8 hours; (0,32 ECTS)
5. Balance of practical student workload:
- participation in tutorial – 10 hours;
Balance of practical student workload is 10 hours which corresponds to 0,4 ECTS
7. Time required to complete the compulsory practice:
Not applicable.
Total: 25 hours (1 ECTS)
Learning outcomes - knowledge
Student:
W1: discusses the fundamental issues related to biology and stem cell
function (B.W19).
W2: characterizes the sources of stem cells of an adult organism
(A.W5); (B.W19); (C.W42).
W3: Lists the basic techniques of cell culture, including isolation and
establishment of primary culture, cell proliferation in in vitro
culture conditions (B.W29); (C.W42).
W4: describes the principles of conduction experimental research in the
field of tissue engineering for medicine development (B.W29);
(C.W42).
Learning outcomes - skills
Student:
U1: Can isolate and establish a primary culture of mesenchymal stromal
stem cells (B.U13).
U2: Can create a spatial culture of cells (B.U13).
U3: Can collect biological material for the isolation and culture of cells
in vitro (B.U13).
U4: Recognizes his own limitations, performs self-evaluation
deficits and educational needs in the field of tissue engineering
(B.U11).
U5: Critically analyzes medical literature and draws conclusions based
on the literature (B.U11).
Learning outcomes - social competencies
Student:
K1: understands the significance of own limitations and ability to pursue
continuing education (K_K07).
K3: can cooperate with representatives of other healthcare professions
(K_K06)
Teaching methods
Tutorial:
• work in laboratory
• didactic discussion,
• case reports
• designs and analysis of scientific research
Type of course
elective course
Course coordinators
Assessment criteria
Assessment methods:
- practical test (0-10 points): U1-U5
Credit based on the appropriate number of points (0-10 points > 60%)
- oral test (0-15 points); the condition of passing the test is to obtain 60% of points: W1-W4; U1-U5.
<8 failed
≥8 credit
Extended observation (0 – 10 points; >50%): K1 – K2
Practical placement
Bibliography
Obligatory:
1. Principles of Regenerative Medicine. 3rd edition. red. A Atala, R.
Lanza, A.G. Mikos, R. Nerem. Academic Press, 2019.
Optionall:
2. Culture of Animal Cells. red. R.I. Freshney, Wiley-Liss, 2009.
3. Cell and Tissue Culture for Medical Research. red. A. Doyle
J.B. Griffiths, John Willey&Sons, 2000.
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: