Biochemistry 1655-Lek11BCHE-J

Lectures:
1. Protein amino acids, characteristics. Structure and properties of peptide bond. Structure and function of biologically important peptides. Insulin biosynthesis and structure.
2 Proteins - classification, characteristics of the 1st, 2nd, 3rd and 4th order structure. Post-translational modifications of proteins. Structure of a ribonuclease. Structure and synthesis of collagen.
3. The relationship between the structure of a protein and its function - myoglobin, hemoglobin, prions, immunoglobulins. The physiological role of hemoglobin and myoglobin. The mechanism of combining oxygen with myoglobin and hemoglobin. Changes in the structure of hemoglobin occurring at various stages of human development. Glycosylated hemoglobin. Hemoglobinopathies.
4. Functions performed by blood. Organic and inorganic components of plasma. Characteristics and functions of plasma proteins. Buffering properties of blood - carbonate buffer and the role of hemoglobin in buffering (Bohr and Halden effect).
5. Enzyme - structure, features, methods of forming enzyme-substrate complexes. The concept of the isoenzyme. Physical and chemical properties of isoenzymes. The importance of isoenzymes in diagnostics. Classification of enzymes.
6. Kinetics of the Michaelis-Menten enzymatic reaction and the allosteric enzyme.
7. Regulation of enzyme activity. Types of inhibition with examples of inhibitors used in medicine.
8. Water-soluble vitamins - structure and importance in metabolic pathways. Structure of coenzymes and functions performed by coenzymes in enzymatic reactions.
9. Fat-soluble vitamins - structure and importance in metabolic pathways and hormonal regulation.
10. The role of enzymes in the digestion of carbohydrates, lipids, proteins and nucleic acids. Composition and role of digestive juices. Role of bile acids in digestive process. Synthesis and enterohepatic circulation of bile acids. Molecular mechanisms of the absorption of digestion products.

Tutorials:
1. Qualitative analysis of amino acids and proteins.
2. Quantitative analysis of proteins.
3. Qualitative and quantitative analysis of blood components.
4. Disorders of protein synthesis and structure in the pathogenesis of selected diseases.
5. Isolation of proteins from biological material.
6. Enzymatic kinetics.
7. Qualitative and quantitative analysis of selected vitamins.
8. The importance of enzymes in the diagnosis and treatment of selected diseases.
9. Selected properties of digestive juices.
10. Repetition 1.

Total student workload

1. The workload related to the classes requiring the direct participation of academic teachers is: - participation in lectures: 25 hours - participation in tutorials: 30 hours - completion of the test: 2 hours The workload related to the activities requiring the direct participation of academic teachers is 57 hours, which corresponds to 2,28 ECTS points 2. The student workload: - participation in lectures: 25 hours - participation in tutorials: 30 hours - preparation for tutorials (including reading the indicated literature): 45 hours - preparation of protocols on tutorials: 9,5 hours - preparation for passing and passing: 26 + 2 = 28 hours The total student workload is 112,5 hours, which corresponds to 4,5 ECTS points 3. The workload related to the implementation of learning outcomes in medical simulation conditions: not applicable 4. The workload associated with achievement of learning outcomes related to medical communication: not applicable

Learning outcomes - knowledge

W1: presents the structure of amino acids and their physical and chemical properties - B.W10. W2: presents the structure of carbohydrates and their physical and chemical properties - B.W9. W3: presents the structure of lipids and their role at the cellular and extracellular level - B.W9. W4: presents structure of polysaccharides and their role at the cellular and extracellular level - B.W9. W5: describes I-, II-, III- and IV-order structure of proteins - B.W10. W6: post-translational and functional modifications of proteins and their role in the functioning of the organism - B.W10. W7: describes the characteristics of proteins involved in the structure of chromatin - B.W11. W8: describes the role of enzymes and vitamins in the course of metabolic processes - B.W13. W9: describes regulatory mechanisms of metabolic processes - B.W13. W10: describes the influence of a proper diet, digestion and absorption processes on the course of metabolic pathways - B.W15. W11: describes the antioxidant role of vitamins - C. W39. W12: describes the role of blood in maintaining the body's water and electrolyte balance - B.W1. W13: describes the participation of blood buffers in maintaining systemic homeostasis - B.W2. W14: presents the chemical composition of digestive juices in the human digestive tract, mechanisms of action of digestive system enzymes and biochemical basis for the absorption of digestive products - B.W15.

Learning outcomes - skills

U1: calculates the molar and percentage concentrations of the dilution solutions - B.U3. U2: determines the pH of the solution and determines the effect of pH changes on the properties of proteins and carbohydrates - B.U5. U3: uses the methods of qualitative and quantitative analysis, titration, spectrophotometry, pH-metry, protein electrophoresis, analyzes and interprets the obtained results - B.U12. U4: operates the analytical balance, spectrophotometer, pH-meter and evaluates the accuracy of the measurements - B.U12. U5: determines pH of body fluids and determines the influence of pH changes on their properties - B.U5.

Learning outcomes - social competencies

K1: shows ability to use reliable sources of scientific information in the field of biochemical knowledge - K_K07. K2: presents the awareness of the limitations of the scope of their knowledge and awarness of the need to constantly supplement it - K_K05. K3: shows the need and the ability to constantly supplement one's knowledge - K_K05. K4: presents the ability to formulate conclusions based on the measurements of biological parameters in biological material - K_K08.

Teaching methods

Lectures: teaching methods - informative lecture, searching teaching methods - didactic discussion Seminars: teaching methods – lecture, problem teaching methods - problem method, didactic discussion, case method, practical didactic methods - project method Tutorials: teaching methods - explanation, problem teaching methods - problem method, didactic discussion, practical teaching methods - laboratory tests, practical exercises, demonstration

Observation/demonstration teaching methods

- display

Expository teaching methods

- informative (conventional) lecture
- problem-based lecture

Exploratory teaching methods

- classic problem-solving
- presentation of a paper
- laboratory
- practical
- experimental
- seminar

Type of course

compulsory course

Prerequisites

A student starting education in the subject of Biochemistry should have knowledge of chemistry and biology at the secondary school level (advanced level of the Matura exam in both subjects).

Course coordinators

Karolina Szewczyk-Golec

Assessment criteria

Lectures:
1. Mid-term test (MCQ) (> 60%): W1-W14, U5, K1.
2. Extended observation (> 50%): K1-K3.
Tutorials:
1. Mid-term test (MCQ) (> 60%): W1, W5-W14, U2, K1, K4.
2. Oral or written answer (> 60%): W1, W5-W14, K1, K4.
3. Directed observation of student activities during practical tasks (> 60%): U1-U4.
4. Report (> 60%): W1, W5-W13, U1-U4, K1, K4.
5. Oral presentation (> 60%): W1, W5-W14, K1-K3.
6. Extended observation (> 50%): K1-K4.

The student will receive a credit for the course, obtaining positive results from two mid-semester tests and a positive assessment of social competences (appropriate number of points from all assessed criteria).

3. Oral or written answer (evaluation of active participation and preparation for the classes): W1-W26, U1-U7, K1-K4.
In order to pass, the student must obtain a minimum of 60% of the answers (0-10 points).

In the case of an oral or written answer, the following criteria are used to assess the learning outcomes achieved by the student:
- pass when: the student knows the basic issues and mastered the minimum curriculum, understands the questions asked, presents his knowledge in a logical and systematic way, is able to apply the acquired knowledge in practice;
- failure to pass the credit if: the student has not mastered the minimum curriculum, does not understand the questions, gives answers off topic, does not use the basic vocabulary correctly, is not able to apply the acquired knowledge in practice.

4. Directed observation of student activities during practical tasks: U1-U6.
In order to pass, the student must obtain a minimum of 60% of points for a correctly performed activity (0-10 points).

5. Protocol: W1-W26, U1-U7, K1, K4.
In order to pass, the student must obtain a minimum of 60% of the points for the presented protocol on the tutorials (0-10 points).

6. Oral presentation: W1-W26, K1-K3.
In order to pass, the student must obtain a minimum of 60% of points for the preparation and presentation of the paper (0-10 points).

7. Activity - extended observation: K1-K4.
In order to pass, the student must obtain at least 50% (0-10 points).

The condition for taking the exam is to obtain a positive result in the mid-semester tests, pass practical tasks, protocols and oral presentations, and obtain a positive assessment of social competences.

Practical placement

not applicable

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 1655-Lek11BCHE-J in USOSweb