(in Polish) Metody separacyjne 0600-S2-O-MS

Lectures:
Introduction to separation methods; Sample preparation methods; Enrichment and extraction techniques; Organic compounds analysis in biological and environmental samples; Physicochemical principles of separation methods; Optimization of separation process; Kinetic theory; Chromatographic plate theory; Apparatus for gas chromatography (GC); Overview and types of detectors and injectors; Liquid chromatography; Equipments for high performance liquid chromatography (HPLC); Injectors, pumps and detectors;
Thin layer chromatography (TLC); Speration process, detection and identification; Mass spectrometry and separation methods.
Laboratory experiments:
1. Identification of organic compounds by high-performance liquid chromatography (HPLC).
2. Quantitative analysis by means of high-performance liquid chromatography (HPLC).
3. Analysis of inorganic anions by ion chromatography (IC).
4. Identification of organic compounds using gas chromatography (GC).
5. Determination of chlorinated compounds by gas chromatography.
6. Comparative analysis of dyes by thin layer chromatography (TLC)
7. Application of solid phase extraction (SPE) and thin layer chromatography (TLC) in the analysis of organic compounds.
8. Extraction and analysis of drugs in biological samples by means of solid phase extraction and determination by liquid chromatography.
9. Determination of ions by isotachophoresis

Total student workload

Hours with teacher: - participation in lectures - 20 hrs - participation in laboratories – 40 hrs - consultations - 5 hrs Self-study hours: - writing reports- 10 hrs - preparation for test/ examination - 35 hrs

Learning outcomes - knowledge

W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01 W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14

Learning outcomes - skills

U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11. U02: make measurements - K_U11. U03: prepare on their own preparations for the tests, analytical procedures - K_U14. U04: can analyze, interpret and calculate the results obtained in the laboratory, in the course of his work - K_U11. U05: can develop analytical methodology - K_U14

Learning outcomes - social competencies

K01: draws up research reports and notes that can be the basis for the preparation of a scientific publication - K_K07 K02: describes and compares the obtained test results with the standards (pattern libraries) and the available literature - K_K01, K_K02

Teaching methods

Lectures Laboratory experiments

Prerequisites

General issues in the field of sample preparation metods and advanced chromatographic and electromigration techniques.

Course coordinators

Tomasz Ligor

Assessment criteria

To pass of laboratory experiments is the knowledge of analytical procedures required for the correct execution of exercises-laboratory
analysis, a positive evaluation of the colloquium preceding the exercise include the written report for each experiments – W01, W02, K02
fail - 2 pts (≥50%)
satisfactory - 3 pts (50-60%)
satisfactory plus - 3,5 pts (61-65%)
good - 4 pts (66-75%)
good plus - 4,5 pts (76-80%)
very good - 5 pts (≤81%)

Bibliography

Scott R.P. W. , Tandem techniques, J. Wiley & Sons, New York 1998.
Poole C.F. , The Essence of Chromatography, Elsevier, Amsterdam 2003.
Pawliszyn J. [Ed.], Sampling and sample preparation for field and laboratory Elsevier Science B.V., Amsterdam 2002
Buszewski B., Dziubakiewicz M., Szumski M. [red.], Electromigration techniques, Theory and Practice, Springer, Berlin 2013.
Ardrey B., Liquid chromatography-mass spectrometry: an introduction, John Wiley & Sosns Inc., Chichester 2003.
Baranowska I., Buszewski B., Bioanalytics. Elsevier, Amsterdam 2022.
Buszewski B., Szultka M., Gadzała-Kopciuch R., Theory of extraction techniques, in: Comprehensive sampling and sample
preparation, J. Pawliszyn (ed.) Academic Press, New York 2012, str. 243-256.
Buszewski B., Szultka M., Past, Present, and Future of Solid Phase Extraction: A Review. Critical Reviews in Analytical Chemistry,
42:3 (2012) 198-213.
Kealey D., Haines P.J., Krótkie wykłady, Chemia Analityczna. PWN, Warszawa 2006.
Skoog D.A., West D.M., Holler J.F., Crouch S.R., Podstawy chemii analitycznej T. 2, przekład zbiorowy A. Hulanickiego [red.], Wydawnictwo Naukowe PWN, Warszawa 2007.
Thurman E. M., Mills M. S., Solid Phase Extraction. Principles and Practice. Wiley, Nowy Jork 1998.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S2-O-MS in USOSweb