Hyphenated Separation Techniques
0600-S2-EN-HST
Sample preparation using solid phase extraction (SPE) and quantitative analysis by high performance liquid chromatography (HPLC).
Application of GC/MS coupled technique.
Separation of nanoparticles by field-flow fractionation - FFF.
Determination of inorganic ions by ion chromatography.
The determination of volatile organic compounds (VOCs) by use of SPME-GC, P&T-GC and LLE-GC methods.
The comparison of HPLC and UHPLC techniques.
Miniaturized separation techniques: capillary liquid chromatography (nanoLC) and capillary electrophoresis.
Total student workload
Lecture:
Total student workload Contact hours with teacher:
np.
- participation in lectures - 30 hrs
- consultations - 15 hrs
Self-study hours:
np.
- preparation for lectures - 15 hrs
- writing essays/ papers/ projects - 5 hrs
- reading literature - 5 hrs
- preparation for test/ examination - 30 hrs
Altogether: 100 hrs (7 ECTS)
Laboratory:
Contact hours with teacher:
np.
- participation in lectures - 30 hrs
- consultations - 15 hrs
Self-study hours:
np.
- preparation for lectures - 15 hrs
- writing essays/ papers/ projects - 5 hrs
- reading literature - 5 hrs
- preparation for test/ examination - 30 hrs
Altogether: 100 hrs (7 ECTS)
Learning outcomes - knowledge
Lecture:
W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01
W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14
Laboratory:
W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01
W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14
Learning outcomes - skills
Lecture:
U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11.
U02: make measurements - K_U11.
U03: prepare on their own preparations for the tests, analytical procedures - K_U14.
U04: can analyzes, interprets and calculates the results obtained in the laboratory, in the course of his work - K_U11.
U05: can develop analytical methodology - K_U14
Laboratory:
U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11.
U02: make measurements - K_U11.
U03: prepare on their own preparations for the tests, analytical procedures - K_U14.
U04: can analyzes, interprets and calculates the results obtained in the laboratory, in the course of his work - K_U11.
U05: can develop analytical methodology - K_U14
Learning outcomes - social competencies
Lecture:
K01: apply appropriate analytical procedures - K_K02.
K02: draw up test reports and notes, which can be used as a basis for the preparation of scientific publications - K_K05.
Laboratory:
Learning outcomes - social competencies K01: apply appropriate analytical procedures - K_K02.
K02: draw up test reports and notes, which can be used as a basis for the preparation of scientific publications - K_K05.
Teaching methods
Expository teaching methods:
- informative lecture
- lectures with discussion
Laboratory experiments
Prerequisites
General issues of advanced chromatographic and electromigration techniques
Course coordinators
Assessment criteria
To pass of laboratory experiments is the knowledge of analytical procedures required for the correct execution of exercises-laboratory analysis, a positive evaluation of the colloquium preceding the exercise include the written report for each experiment – W01, W02, K02
fail - 2 pts (≥50%)
satisfactory - 3 pts (50-60%)
satisfactory plus - 3,5 pts (61-65%)
good - 4 pts (66-75%)
good plus - 4,5 pts (76-80%)
very good - 5 pts (≤81%)
Practical placement
Work placement not applicable
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: