Hyphenated Separation Techniques
0600-S2-EN-HST
Sample preparation using solid phase extraction (SPE) and quantitative analysis by high performance liquid chromatography (HPLC).
Application of GC/MS coupled technique.
Separation of nanoparticles by field-flow fractionation - FFF.
Determination of inorganic ions by ion chromatography.
The determination of volatile organic compounds (VOCs) by use of SPME-GC, P&T-GC and LLE-GC methods.
The comparison of HPLC and UHPLC techniques.
Miniaturized separation techniques: capillary liquid chromatography (nanoLC) and capillary electrophoresis.
Total student workload
Lecture:
Total student workload Contact hours with teacher:
np.
- participation in lectures - 30 hrs
- consultations - 15 hrs
Self-study hours:
np.
- preparation for lectures - 15 hrs
- writing essays/ papers/ projects - 5 hrs
- reading literature - 5 hrs
- preparation for test/ examination - 30 hrs
Altogether: 100 hrs (7 ECTS)
Laboratory:
Contact hours with teacher:
np.
- participation in lectures - 30 hrs
- consultations - 15 hrs
Self-study hours:
np.
- preparation for lectures - 15 hrs
- writing essays/ papers/ projects - 5 hrs
- reading literature - 5 hrs
- preparation for test/ examination - 30 hrs
Altogether: 100 hrs (7 ECTS)
Learning outcomes - knowledge
Lecture:
W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01
W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14
Laboratory:
W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01
W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14
Learning outcomes - skills
Lecture:
U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11.
U02: make measurements - K_U11.
U03: prepare on their own preparations for the tests, analytical procedures - K_U14.
U04: can analyzes, interprets and calculates the results obtained in the laboratory, in the course of his work - K_U11.
U05: can develop analytical methodology - K_U14
Laboratory:
U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11.
U02: make measurements - K_U11.
U03: prepare on their own preparations for the tests, analytical procedures - K_U14.
U04: can analyzes, interprets and calculates the results obtained in the laboratory, in the course of his work - K_U11.
U05: can develop analytical methodology - K_U14
Learning outcomes - social competencies
Lecture:
K01: apply appropriate analytical procedures - K_K02.
K02: draw up test reports and notes, which can be used as a basis for the preparation of scientific publications - K_K05.
Laboratory:
Learning outcomes - social competencies K01: apply appropriate analytical procedures - K_K02.
K02: draw up test reports and notes, which can be used as a basis for the preparation of scientific publications - K_K05.
Teaching methods
Expository teaching methods:
- informative lecture
- lectures with discussion
Laboratory experiments
Prerequisites
General issues of advanced chromatographic and electromigration techniques
Course coordinators
Term 2023/24L: | Term 2024/25L: | Term 2025/26Z: |
Assessment criteria
To pass of laboratory experiments is the knowledge of analytical procedures required for the correct execution of exercises-laboratory analysis, a positive evaluation of the colloquium preceding the exercise include the written report for each experiment – W01, W02, K02
fail - 2 pts (≥50%)
satisfactory - 3 pts (50-60%)
satisfactory plus - 3,5 pts (61-65%)
good - 4 pts (66-75%)
good plus - 4,5 pts (76-80%)
very good - 5 pts (≤81%)
Practical placement
Work placement not applicable
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: