Selected imaging techniques of chemical and biological systems 0600-S2-CM-PW-TOUCB

The subject of the lecture concerns the basic issues related to the preparation of chemical systems (e.g. metal nanoparticles) and biological systems (cells, tissues) for imaging using optical and electron microscopy. The content will cover the selection of the method of preserving biological material and chemical systems, the effectiveness of drainage and resin supersaturation, the selection of resins for a specific application, polymerization conditions. Students will be familiarized with the techniques of preparing glass knives, using an ultramicrotome for the preparation of ultra- and semi-thin sections, and with appropriate contrasting and staining for observations at the level of electron and optical microscopy. Students will make their own preparations for analysis using scanning electron microscopy and transmission electron microscopy.
Students will be familiarized with the techniques of preparing biological material for analysis using a Raman confocal dispersion spectrometer. The students' task will also be to familiarize themselves with the description of the obtained images as well as their interpretation.

Total student workload

1. 10 h lecture; 20 h laboratory classes, 35 h consultations 2. 10 h preparation for laboratory classes; 15 h preparation of theoretical and experimental reports; 10 h preparation of the thesis to be completed Total: 100 hours (4 ECTS)

Learning outcomes - knowledge

W1: The student knows the theoretical and practical aspects of imaging using optical and electron microscopy and the principles of operation of microscopes (K_W01, K_W03) W2: The student knows the basics of the methodology of preparing samples for analysis, including fixation, drainage and embedding in resin W3: The student knows the techniques of making semi-thin sections for the initial assessment of the material using optical microscopy, and then ultra-thin sections for analysis using transmission electron microscopy, preparation of glass knives, staining and contrasting W4: The student knows the techniques of preparing biological material for analysis using a Raman, confocal dispersion spectrometer (K_W01, K_W03, K_W04)

Learning outcomes - skills

U1: The student has the ability to select the technique of preparing chemical and biological material for the planned imaging method (K_U01, K_U02) U2: The student is able to prepare samples (fix, sink and cut) and analyze them (K_U03) U3: can correctly interpret the obtained images (K_U04)

Learning outcomes - social competencies

K1: The student is aware of the importance of the proper preparation of the sample for microscopic analysis K2: The student is prepared to put into practice the knowledge gained during lectures and laboratory classes K3: The student is aware of the acquired knowledge and skills, is able to complete and improve them K4: Can systematically and independently work in order to properly complete a task K5: Can cooperate in a team and is responsible for the tasks entrusted to him K6: Creatively solves problems and draws conclusions K_K01, K_K03

Teaching methods

Lecture: lecture with multimedia presentation Laboratory: performing experiments. The student performs the exercises in the laboratory individually or in pairs according to the available instructions, suggestions and instructions from the teacher. Consultations, discussion on the purposefulness of the activities performed

Expository teaching methods

- problem-based lecture
- informative (conventional) lecture

Exploratory teaching methods

- observation
- experimental
- laboratory

Online teaching methods

- exchange and discussion methods

Type of course

elective course

Prerequisites

Basic course in biology and analytical chemistry

Course coordinators

Katarzyna Rafińska

Assessment criteria

Assessment methods:
Lecture:
Credit on the basis of theoretical work on a selected issue in imaging techniques
Lab:
Credit covering activity in the classroom, quality of work
laboratory and a written presentation of the obtained results
(reports)
Assessment criteria
Lecture:
Work on the basis of the available textbooks in Polish - sufficient
Work based on Polish-language publications - good
Work on the basis of English-language publications and textbooks - very good
Lab:
grading based on the number of points from the reports
(the quality of laboratory work, the way of conducting experiments, as well as the ability to cooperate in a group will be assessed). The required threshold for a satisfactory grade - 50-60%, satisfactory plus - 61-65%, good - 66-75%, good plus - 76-80%, very good - 81-100%.

Practical placement

not applicable

Bibliography

Basic literature:
•Barbacki A. (red.) Mikroskopia Elektronowa. Wydawnictwo Politechniki Poznańskiej, Poznań, 2007
• Litwin J.A., Gajda M., Podstawy technik mikroskopowych, Wydawnictwo Uniwersytetu Jagielońskiego, Kraków, 2011
• Wróbel B., Zienkiewicz K., Smoliński D.J., Niedojadło J., Podstawy mikroskopii elektronowej. Skrypt dla studentów biologii, Wydawnictwo Uniwersytetu Mikołaja Kopernika, Toruń, 2005
Supplementary literature:
• Dziadur W., Mikuła J., Mikroskopia elektronowa. Tom I. Mikroskopia Transmisyjna, Wydawnictwo Politechniki Krakowskiej, Kraków, 2014•Dziadur W., Mikuła J., Mikroskopia elektronowa. Tom II. Mikroskopia Skaningowa, Wydawnictwo Politechniki Krakowskiej, Kraków, 2016

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S2-CM-PW-TOUCB in USOSweb