Identification and determination of active pharmaceutical ingredients by microscopic-spectroscopic methods 0600-S2-CM-PW-IOSA

The subject consists of a series of lectures (10 hours) and workshops (20 hours).
The lecture presents the basics and application of instrumental methods, focused around combined techniques of microscopy and spectroscopy. These are two groups of methods that have developed in a revolutionary way in recent years and are used in modern analytical chemistry to identify and determine solid substances and their impurities.
The experimental part of the course is planned in the laboratory of electron microscopy (SEM-EDS), optical microscopy (MO) and IR and Raman spectroscopy. The classes consist of three tasks. The first involves the identification of selected patterns of active substances (e.g. paracetamol, ibuprofen, acetylsalicylic acid, naprofen, caffeine) and selected excipients (e.g. glucose, fructose, sucrose). The second task concerns the identification of reference substances from the first task in mixtures. The third task is to quantify the active substances in tablets using the standard curve method.

Total student workload

Time implemented with the participation of teachers (65 h): - participation in lectures – 10 h - participation in laboratory – 20 h - consultations and work with an academic teacher on the preparation of a laboratory report - 35 hours. The time spent on individual student work (35 h): - preparation for the laboratory - 10 hours. - preparation for the lecture - 5 hours. - reading literature – 5 godz. - prepare the student for the exam - 15 hours. Total: 100 hours (4 ECTS)

Learning outcomes - knowledge

Student: W1: Has knowledge of light and electron imaging as well as molecular and X-ray spectroscopy. W2: Knows and understands the relationship between light and electron imaging and spectroscopic analysis. W3: Knows the basics of light and electron microscopy as well as molecular and X-ray spectroscopy; including specialist nomenclature. W4: Describes the various types of microscopy and spectroscopy, their properties and practical applications. W5: Characterizes the properties of chemical compounds on the basis of the image and spectrum; can use the terms used in medical chemistry. W6: Knows the latest developments in the field of microscopy and spectroscopy and their combined methods (correlation microscopy).

Learning outcomes - skills

Student: U1: Consolidates the basics of chemical knowledge acquired at the previous stages of education, basic definitions, chemical nomenclature. Uses the acquired knowledge to critically analyze the results obtained with the methods of instrumental analysis. U2: Critically analyzes the acquired knowledge in the field of instrumental methods, proposes appropriate solutions to solve the problems posed. U3: Can independently prepare a report on the use of light microscopy, electron microscopy, molecular and X-ray spectroscopy for identification and determination of pure substances and in their mixtures.

Learning outcomes - social competencies

Student: K1: Works independently and efficiently with large amounts of information. K2: Thoughts creatively in order to improve the existing or create new solutions. K3: Knows the limitations of his own knowledge and skills. K4: Establishes cooperation in a group.

Teaching methods

Teaching methods giving: - lecture (conventional) using multimedia presentations. Teaching methods searching: - laboratory are related to the program content of the lectures. The student performs tasks in pairs, based on the available instructions and the recommended literature. The results of the measurements are prepared by the student in the form of a report on the completed task, which is the basis for the assessment of the laboratory performance.

Expository teaching methods

- informative (conventional) lecture

Exploratory teaching methods

- laboratory

Type of course

elective course

Prerequisites

absence

Course coordinators

Grzegorz Trykowski

Assessment criteria

Evaluating methods:
lecture – exam W1, W2, W3, W4, W5, W6
laboratory – report U1, U2, U3, K1, K2, K3, K4
Assessment criteria:
Lecture:
Block assessment with the following weights:
- 50% exam
- 50 % evaluation of laboratory
Threshold required to assess:
- dostateczną (3): 50 -60 %
- dostateczną plus (3+): 61 – 65 %
- dobrą (4): 66 – 75 %
- dobrą plus (4+): 76 – 80 %
- bardzo dobrą (5): 81-100 %
Laboratory:
Grading based on:
- prepared reports based on the results
Threshold required to assess:
- dostateczną (3): 50 -60 %
- dostateczną plus (3+): 61 – 65 %
- dobrą (4): 66 – 75 %
- dobrą plus (4+): 76 – 80 %
- bardzo dobrą (5): 81-100 %

Bibliography

1.Dziadur W., Mikuła J., Mikroskopia elektronowa, t. 3: Praktyczna mikroskopia skaningowa: wybrane zastosowania w inżynierii materiałowej, Politechnika Krakowska, Kraków 2019.
2.Trykowski G., Badania nanomateriałów metodami mikroskopii elektronowej, Wydawnictwo Naukowe UMK, Toruń 2022.
3.Sadlej J., Spektroskopia molekularna, Wydawnictwo Naukowo-Techniczne, Warszawa, 2002.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S2-CM-PW-IOSA in USOSweb