Photochemistry and free radicals 0600-S2-CM-FWR

Lecture:
The lecture contains information on the nature of electromagnetic radiation and the interaction of light with matter. Basic concepts and laws of photochemistry, issues related to radiation absorption and excited states of molecules and excited state quenching processes, fluorescence and phosphorescence, Jabłoński diagram, quantum yields, energy and electron transfer processes, reactive oxygen species will be discussed. Examples of photochemical reactions will be presented. The use of photochemistry in medicine.
Types and sources of free oxygen radicals and free radicals with an unpaired electron and reactive oxygen species; mechanisms of free radicals formation and their influence on organisms - positive and negative aspects; application of active radicals and reactive oxygen species in medicine, and methods for determining free radicals and antioxidant activity will be discussed.

Lab:
Laboratory exercises are devoted to the detection of singlet oxygen, the study of the influence of UV radiation on the decomposition of dye in the presence and absence of semiconductors, the study of photochromic processes in azo compounds, the study of photochemical transformations of pyrimidine and purine bases, and the study of photosensitization and photostabilization processes, determination of free radicals and antioxidant activity, analysis of the effect of UV radiation on the content of free radicals and antioxidants

Total student workload

1. 10 hours of lecture, 30 hours of laboratory, 25 hours of consultations, i.e. 65 hours of contact 2. 20 hours of individual student work, 3. 15 hours of time required to prepare to participate in the assessment process, 4. total student workload - 10 hours of material, writing papers, projects, reading literature

Learning outcomes - knowledge

student: W1: knows the influence of electromagnetic radiation on matter - K_W01 W2: distinguishes photophysical and photochemical processes - K_W01 W3: knows the basic photochemical laws - K_W01 W4: defines the quantum efficiency of energy and electron transfer processes and quenching processes - K_W01 W5: knows the sources of UV radiation - K_W01, K_W03 W6: knows the types, chemical structure, role and mechanisms of action of free radicals and reactive oxygen species and antioxidants – K_W01, K_W W7: knows the analytical methods for determination of antioxidants and radicals as well as antioxidant capacity and antiradical activity – K_W01, K_W03

Learning outcomes - skills

student: U1: performs simple photochemical experiments - K_U01 U2: correctly interprets the effects of UV radiation on matter - K_U01, K_U03 U3: safely uses UV radiation sources – K_U03 U4: can measure the intensity of UV radiation using chemical actinometers - K_U01 U5: describes the sources of free radical generation, the mechanisms of their formation and the effects of their action – K_U01 U6: links the chemical structure of an antioxidant with its antioxidant potential – K_U01 U7: selects the appropriate analytical method for determining free radicals and antioxidant activity – K_U03

Learning outcomes - social competencies

student: K1: independently sees relationships and draws conclusions correctly - K_K01, K2: works systematically and consistently - K_K01. K3: knows and follows the rules and standards applicable in the photochemistry laboratory - K_K01, K4: works in a group – K_03 K5: focuses on acquiring new knowledge, skills and experiences regarding reactive oxygen species, free radicals, free radical processes and oxidation and the role of antioxidants – K_K01

Teaching methods

Lecture: Lecture with multimedia presentations Lab: Independent laboratory work

Expository teaching methods

- informative (conventional) lecture

Exploratory teaching methods

- observation
- experimental
- laboratory

Type of course

compulsory course

Prerequisites

Basic knowledge of general and organic chemistry

Course coordinators

Term 2023/24L:

Jolanta Kowalonek
Aleksandra Szydłowska-Czerniak

Term 2022/23L:

Halina Kaczmarek
Aleksandra Szydłowska-Czerniak

Term 2025/26L:

Jolanta Kowalonek
Aleksandra Szydłowska-Czerniak

Term 2024/25L:

Jolanta Kowalonek
Aleksandra Szydłowska-Czerniak

Assessment criteria

Lecture: written exam – W1-W5.
Laboratory: credit based on completed laboratory exercises and studies - U1 - U4, continuous assessment of the student's work during classes - U3, U4, K1, K3, K4

Practical placement

not applicable

Bibliography

List of basic literature:
1. S. Paszyc, Basics of photochemistry, PWN, Warsaw 1992.
2. P. Suppan, Chemistry and light, PWN, Warsaw 1997.
3. Collective work, J. Pączkowski (ed.), Photochemistry of polymers, Nicolaus Copernicus University Publishing House, Toruń 2003.
4. Collective work, J. Najbar, A. Turek (eds.), Photochemistry and optical spectroscopy. Laboratory exercises, PWN, Warsaw 2009.
5. W. Schnabel, Polymers and Light. Fundamentals and Technical Applications, Wiley-VCH, Weinheim 2007.
4. Włodzimier Grajek, Przeciwutleniacze w żywności. Aspekty zdrowotne, technologiczne, molekularne i analityczne, Wydawnictwo Naukowo-Techniczne, Warszawa, 2007
5. Grzegorz Bartosz, Druga twarz tlenu. Wolne rodniki w przyrodzie, Wydawnictwo Naukowe PWN, 2013
List of additional literature:
1. Collective work, B. Marciniak (ed.), Methods of investigating the mechanisms of photochemical reactions, Wydawnictwo UAM, Poznań 1999.
2. G. Wenska, Environmental photochemistry, Wydawnictwo UAM, Poznań 1997.
3. Z. Stasicka, Photochemical processes in the environment, Wydawnictwo UJ, Kraków 2001.
4. Yunbo Li, Antioxidants in Biology and Medicine: Essentials, Advances, and Clinical Applications. Nova Science Publishers Inc., New York, 201

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S2-CM-FWR in USOSweb