Physicochemical methods of separation in medicine and pharmacy 0600-S2-CM-FMRMF

Lecture:
1. separation of mixtures
2. distillation
3. adsorption processes
4. basic extraction methods
5. advanced extraction techniques
6. chromatographic techniques
7. preparative scale liquid chromatography
8. membrane separation
9. electromigration techniques
10. practical aspects of mixtures separation
Lab:
Isolation of volatile phenols from hospital wastewater
Thin layer chromatography in the determination of isomers of biologically active compounds
Purification and fractionation of plant extracts with
solid phase extraction
Application of gas chromatography in the analysis of pollutants
pharmaceutical packaging
Investigation of the kinetics of salicylic acid adsorption from aqueous solutions on activated carbon
Study of the isotherm of ibuprofen adsorption from aqueous solutions on activated carbon
Study of the adsorption of salicylic acid from aqueous solutions on activated carbon under dynamic conditions
Isolation and quantification of proteins of animal origin
Determination of casein by the biuret method and study of the effect of solution pH on its solubility
Extraction of vegetable fats using the Soxhlet apparatus

Total student workload

Hours with the participation of teachers: lecture: 10h laboratory: 45h consultations: 35h Self-study hours: preparation for lectures: 20h writing reports: 15h reading literature: 20h preparation for the exam / colloquium: 30h

Learning outcomes - knowledge

W01: uses and explains specialist terminology concerning the subject in Polish - K_W01 W02: recognizes and differentiates issues related to the subject matter of classes on the application of analytical techniques in the determination of biologically active substances - K_W13, K_W14

Learning outcomes - skills

U01: the student applies the acquired theoretical knowledge, including the possibility of using modern analytical apparatus in a correct and proper way - K_U11. U02: the student selects literature sources in a given field - K_U11. U03: the student is able to prepare analytical procedures - K_U14. U04: independently analyzes and interprets research results - K_U11. U05: can prepare complex analytical methodologies - K_U14.

Learning outcomes - social competencies

K01: applies appropriate analytical procedures - K_K02. K02: takes notes that can be the basis for preparing a scientific publication - K_K05.

Teaching methods

Laboratory exercises

Expository teaching methods

- discussion
- participatory lecture

Exploratory teaching methods

- laboratory
- experimental

Prerequisites

General topics in the field of separation methods of multicomponent mixtures. Methods for the isolation, purification, and quantitative analysis of one or more compounds. Chromatographic and spectroscopic techniques.

Course coordinators

Magdalena Ligor

Assessment criteria

The lecture ends with an exam. The passing percentage is 50%.
Laboratory: Obtain a minimum of 50% on entrance colloquia and 50% on written reports after the exercises.

Practical placement

N/A

Bibliography

Literature:
B. Buszewski. E. Dziubakiewicz, M. Szumski [red.]; Techniki elektromigracyjne. Teoria i praktyka. Malamut, Warszawa 2012.
I. Baranowska, B. Buszewski [red.], Bioanalityka w nauce i życiu - tom 1, PWN, Warszawa 2020, wyd. 1. ISBN: 978-83-01-21281-0.
I. Baranowska [red.]; Analiza śladowa. Zastosowania, Wydawnictwo MALAMUT, Warszawa 2013.
Z. Brzózka [red.]; Miniaturyzacja w analityce, Oficyna Wydawnicza Politechniki Warszawskiej, Warszawa 2005.
J.R. Dean, Methods for environmental trace analysis, John Wiley & Sosns Inc., Chichester 2003.
A. Hulanicki, Współczesna chemia analityczna. Wybrane zagadnienia. Wydawnictwo Naukowe PWN, Warszawa 2000.
R. A. W. Johnstone, E. R. Malcolm, Spektrometria mas. Podręcznik dla chemików i biochemików. Wydawnictwo: Wydawnictwo Naukowe PWN, Warszawa 2001.
R. Kellner, J.-M. Mermet, M. Otto, M. Valcarcel, M. Widmer, Analytical Chemistry: A Modern Approach to Analytical Science – 2nd Edition. Wiley-VCH, Weinheim 2004.
R. Kellner, J.-M. Mermet, M. Otto, H. M. Widmer [red], Analytical Chemistry. Wiley-VCH, Weinheim 1998.
D. A. Skoog, D. M. West, J. F. Holler, S. R. Crouch, Podstawy chemii analitycznej. T. 2, przekład zbiorowy pod redakcją A. Hulanickiego. Wydawnictwo Naukowe PWN, Warszawa 2007.
O. D. Sparkman, Z. Penton, F. G. Kitson, Gas Chromatography and Mass Spectrometry: A Practical Guide – 2nd Edition. Academic Press, USA, Massachusetts, Cambridge 2011.
C. F. Poole, S. K. Poole, Chromatography today, Elsevier, Amsterdam 1991.
B. Solnica, K. Sztefko; Medyczne laboratorium diagnostyczne. Metodyka i aparatura. Wydawnictwo Lekarskie PZWL, Warszawa 2015.
P. Konieczka, J. Namieśnik, B. Zygmunt; Ocena i kontrola jakości wyników pomiarów analitycznych, Wydawnictwo WNT, Warszawa 2009.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S2-CM-FMRMF in USOSweb