Chromatographic Analysis 0600-S2-CKR-AC

Lectures:
1 Introduction to chromatographic analysis
2 Physicochemical basics of chromatographic techniques
3 Optimizing the separation process in GC
4 Kinetic theory
5 Apparatus for GC
6 Overview and types of detectors
7 HPLC chromatography
8 TLC chromatography

Lab:
1. Identification of organic shot residues by high-performance liquid chromatography
2. Quantitative analysis of 1,3-diethyl-1,3-diphenylurea on ammunition shells using high-performance liquid chromatography
3. Analysis of inorganic anions in explosives residues
4. Identification and determination of impurities in ethanol using gas chromatography with a flame ionization detector (GC-FID)
5. Determination of chloroform in biological matrices (urine, serum) by gas chromatography
6. Detecting fraudulent documents - comparative analysis of writing materials using thin layer chromatography (TLC)
7. Application of solid phase extraction (SPE) and thin layer chromatography (TLC) in the analysis of drug mimics
8. Analysis of drugs in biological material. Isolation of quetiapine and its biologically active metabolite from plasma by solid phase extraction and determination by liquid chromatography
9. Determination of potassium concentration in plasma samples by isotachophoresis

Total student workload

Contact hours with teacher: - participation in lectures - 15 hrs - participation in laboratories – 60 hrs - consultations - 15h Self-study hours: - preparation for lectures - 15 hrs - writing reports- 25 hrs - reading literature - 10 hrs - preparation for test/ examination - 35 hrs

Learning outcomes - knowledge

W01: uses and explains terminology specific to the subject in English and its equivalent in Polish - K_W01 W02: recognizes and distinguishes between issues related to the subject of classes - K_W13, K_W14

Learning outcomes - skills

U01: apply theoretical knowledge in practice, including the use of facilities and other small laboratory equipment in a correct and proper way - K_U11. U02: make measurements - K_U11. U03: prepare on their own preparations for the tests, analytical procedures - K_U14. U04: can analyze, interpret and calculate the results obtained in the laboratory, in the course of his work - K_U11. U05: can develop analytical methodology - K_U14

Learning outcomes - social competencies

K01: draws up research reports and notes that can be the basis for the preparation of a scientific publication - K_K07 K02: describes and compares the obtained test results with the standards (pattern libraries) and the available literature - K_K01, K_K02

Teaching methods

Lectures Laboratory experiments

Prerequisites

General issues in the field of advanced chromatographic and electromigration techniques.

Course coordinators

Term 2023/24L:

Term 2021/22L:

Term 2022/23L:

Term 2024/25L:

Assessment criteria

To pass of laboratory experiments is the knowledge of analytical procedures required for the correct execution of exercises-laboratory analysis, a positive evaluation of the colloquium preceding the exercise include the written report for each experiments – W01, W02, K02
fail - 2 pts (≥50%)
satisfactory - 3 pts (50-60%)
satisfactory plus - 3,5 pts (61-65%)
good - 4 pts (66-75%)
good plus - 4,5 pts (76-80%)
very good - 5 pts (≤81%)

Bibliography

C. F. Poole, S. K. Poole, Chromatography today, Elsevier, Amsterdam 1991.
R.J. Hamilton, P.A. Sewell, Wysokosprawna chromatografia cieczowa, PWN, Warszawa 1982.
W. Rödel, G. Wölm. Chromatografia gazowa, Wydawnictwo Naukowe PWN, Warszawa 1992.
J.R. Dean, Methods for environmental trace analysis, John Wiley & Sosns Inc., Chichester 2003.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S2-CKR-AC in USOSweb