Bases of the chemistry of biological processes and bioanalytics 0600-S1-O-PChPBB

The subject of the lecture concerns the basic issues related to the knowledge of biological processes and the factors influencing their course. The content will be discussed, i.e.: pro and eukaryotic cells, viruses, structure and functions of the cell membrane, structure of the cell nucleus, processes taking place in the cell nucleus; cell structure; cell cycle; basic biochemical and metabolic concepts; biogenic elements in the structure and metabolism of organisms; amino acids, peptides, proteins, enzymes, nucleic acids - their structure, types, functions, properties; replication, transcription, translation; sugars and fats - structure, types and functions; vitamins, enzymes, coenzymes, their structure, types, biological and metabolic functions; metabolism of carbohydrates, lipids; ATP biosynthesis; processes of DNA synthesis (replication), RNA (transcription) and proteins (translation). Students will also be introduced to instrumental methods used in the analysis of, inter alia, proteins and nucleic acids (from cell lysis methods, through purification, separation, qualitative and quantitative analysis).
Laboratory exercises are designed to prepare for work in research, control and diagnostic laboratories in terms of interpreting results in comparison with standards, identifying biologically active compounds and determining their content. The aim of the laboratory exercises is also to acquire by the student the ability to analyze e.g. sugars, proteins, nucleic acids, enzymes, macronutrients in biological matrices.

Term 2021/22Z:

Term 2022/23Z:

None

Term 2023/24Z:

Total student workload

1.30 h lecture; 30 h laboratory classes, 2. 15 h to prepare for the exam; 10 h preparation for laboratory classes; 10 h preparation of theoretical and experimental reports; 15 h individual work for experimental exercises; 10 h participation in consultations 3.10 h time required for preparation in the assessment process. Total: 130 hours (5 ECTS)

Learning outcomes - knowledge

W1: knows the theoretical and practical aspects of performing qualitative and quantitative analysis using classical and instrumental methods and the principles of apparatus operation W2: knows the basics of biochemistry and the chemistry of metabolic processes W3: knows the techniques of collecting and preparing samples from biological matrices for analysis and toxicity tests W4: knows the rules and principles in the field of occupational health and safety, basic concepts in the field of toxicology; legal acts concerning standards and requirements of chemical laboratories as well as legal regulations concerning hazardous substances and their storage and labeling

Learning outcomes - skills

U1: has the ability to perform measurements of basic chemical quantities and is able to process the results of physico-chemical experiments U2: can prepare samples and analyze them U3: can behave properly in the event of various types of threats, e.g. fire, contact with chemical reagents

Learning outcomes - social competencies

K1: Analytical thinking: Works independently and effectively with a large amount of information, sees the relationships between phenomena and correctly draws conclusions using the principles of logic K2: Creativity: Thoughts creatively to improve existing or create new solutions K3: Conscientiousness and accuracy: Is focused on the best execution of the task; takes care of the detail; Is systematic K4: Perseverance and consistency: Works systematically and has a positive approach to difficulties on the way to achieving the set goal; meets deadlines; understands the need for systematic work on all projects

Teaching methods

Lecture: lecture with multimedia presentation Laboratory: performing experiments. The student performs the exercises in the laboratory individually or in pairs according to the available instructions, suggestions and instructions from the teacher. Consultations, discussion on the purposefulness of the activities performed

Prerequisites

None

Course coordinators

Term 2021/22Z:

Bogusław Buszewski

Term 2022/23Z:

Renata Gadzała-Kopciuch

Term 2023/24Z:

Bogusław Buszewski

Assessment criteria

Assessment methods:
Lecture:
Written exam with open and closed questions
Lab:
Continuous assessment including activity in the classroom, quality of laboratory work and a written presentation of the obtained results (reports), control written tests - determining the final grade as the average of the partial grades obtained during the semester

Assessment criteria:
Lecture: graded exam on the basis of the issues listed in the program content of the subject; answers to the questions will require solving tasks related to the assumed learning outcomes;
The required threshold for the grade:
• for a satisfactory grade: obtaining more than 50% of the points by the student
• with a good mark: obtaining more than 70% of points by the student
• with a very good mark: obtaining more than 81% of points by the student.
Laboratory: credit with a grade on the basis of the number of points from the control tests covering the subject of experiments performed as part of laboratory exercises, performance of all experiments provided for in the program of activities (the quality of laboratory work, the way of conducting experiments, and the ability to cooperate in a group will be assessed) and the analysis of the obtained results in the form of reports.
The required threshold for a satisfactory grade - 50-60%, satisfactory plus - 61-65%, good - 66-75%, good plus - 76-80%, very good - 81-100%.

Practical placement

N/A

Bibliography

Basic literature:
• Berg J.M., Stryer L., Tymoczko J. Biochemistry. WH Freeman 2019
• Hames D.B., Hooper N.M. Bicohemistry. Instant notes. Taylor and Francis Group, New York 2011
• Lundblad R.L., Macdonald F. Handbook of Biochemistry and Molecular Biology. Taylor and Francis Group, Boca Raton
2018
Supplementary literature:
Recommended review articles from Journal citation Report

Term 2021/22Z:

Term 2022/23Z:

None

Term 2023/24Z:

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S1-O-PChPBB in USOSweb