(in Polish) Fizykochemiczne metody badawcze 0600-S1-O-FMB

Complete description of the course Lecture
The aim of the course is to familiarize students with spectroscopic methods used in chemistry (electron spectroscopy of organic compounds, nuclear magnetic resonance, 1H, 13C NMR spectra, IR spectroscopy) and the basic techniques necessary for their practical application. The discussed theoretical issues will illustrate the issues of chemistry. They will help to understand the versatility of the use of spectroscopic methods.
Lectures are organized in such a way that they begin with the presentation of the necessary theoretical foundations of a given phenomenon, followed by examples illustrating its application to the study of specific biochemical problems.

Program content of the lecture
• characteristics of electromagnetic radiation.
• symmetry of molecules and determination of point groups.
• basic issues in group theory.
• probability of transitions and selection rules in spectroscopy.
• symmetry of molecular orbitals in simple organic molecules.
• electron configurations and symmetry of the ground state and excited states.
• physical basics of the nuclear magnetic resonance method (resonance frequencies of magnetic nuclei, relaxation phenomena).
•1H NMR spectroscopy (basic concepts, chemical shift and factors influencing its size and value changes; use of chemical shifts to interpret 1H NMR spectra).
•13C carbon nuclear magnetic resonance spectroscopy (factors influencing chemical shifts; 13C NMR spectrum and the structure of the molecule).
• theoretical basis of vibrational spectroscopy.
• the use of infrared spectroscopy in analytical chemistry.
• comparison of the suitability of various spectroscopic methods for solving structural problems.

Laboratory curriculum:
The course will allow students to acquire the skills of registering, analyzing and interpreting spectra leading to proposing the structure of compounds on the basis of spectroscopic parameters. Practical use of the acquired theoretical knowledge will allow for independent solving of structural problems of macromolecules.
Program content
Laboratory:
1. Introductory laboratory (2h)
2. Interpretation of 1H NMR spectra of organic compounds (chemical shift, spin-spin coupling constant, spin systems). (5h)
3. Simulating and interpreting the 13C NMR spectra of organic compounds. (5h)
4. Introduction to the analysis of vibrational and electronic spectra-group theory. (5h)
5. Application of IR spectroscopy for the qualitative identification of compounds part 1. (5h)
6. Application of IR spectroscopy for the qualitative identification of compounds part 2. (5h)
7. Analysis and registration of electron spectra of organic compounds. (5h)
8. Application of Raman spectroscopy to the interpretation of compounds. (5h)
9. Comprehensive interpretation of the structure of compounds based on the IR, UV-Vis and NMR spectra. (5h)
10. Colloquium (3h).