Biomedical analytics with elements of metabolomics 0600-S1-CM-PW7a-Va

Fundamentals of biological material preparation methods, i.e. blood, plasma, urine, solid tissues for analytical determinations. Modern methods of analyzing biomolecules. Metabolomics. Analytical techniques used in metabolomics. Overview of the development of research in metabolomics in the development of modern science. Application of metabolomics in modern medical diagnostics. Application of distribution methods in biomedical chemistry: liquid, thin-layer and gas chromatography with various detectors, gel and capillary electrophoresis. Apparatus and methods of detection. Special techniques: micellar electrophoresis, chiral analysis, electrochromatography. Examples of applications of chromatography and capillary electrophoresis in the analysis of natural products. Basics of mass spectrometry. Types of ion sources and methods of ionization. Mass analyzers and ion detectors, among others Ion trap, single quadrupole, triple quadrupole, TOF and MALDI TOF analyzers for metabolomics.
Lectures:
Biomedical analytics. Breakdown of sample preparation methods. Modern analytical techniques – application possibilities. Chromatographic techniques - nomenclature, qualitative and quantitative analysis, validation, derivatization methods. Preparation of biological samples for analysis. Microextraction methods in analytics. Applications of electromigration techniques. The concept of metabolomics. Food safety - general issues. Supplementation in the human diet. Volatile organic compounds analytics. Free radicals and antioxidants - division, properties, methods of analysis. Causes and effects of reactive oxygen species. Methods for the determination of antioxidant activity. Analysis of biological samples - tests for medical purposes.

Total student workload

Lectures: 1. Hours carried out with the participation of teachers: contact hours provided for in the study plan for a given subject (the sum of hours of all forms of classes in the subject; in the case of classes carried out with the use of distance learning methods and techniques - the number of contact hours conducted "stationary" and the number of hours conducted in the synchronous model) as well as hours of individual consultations of a student / student / participant of the course (in the case of classes carried out with the use of distance learning methods and techniques - the number of contact hours for "stationary" consultations and the number of hours for on-line consultations) : 10 h Activities: 30 h (lectures and laboratories). Time devoted to individual work of a student / student / participant of the course needed to successfully complete the course, i.e. earlier preparation and completion of notes; collecting and selecting appropriate materials for classes, revision of the material required, writing works, projects, reading literature; 15 h. Time required to prepare for and to participate in the assessment process (e.g. exams): 30 h. Time required for independent practices: 0 h.

Learning outcomes - knowledge

W01: has extensive knowledge of analytical chemistry, its development and importance for the progress of exact and natural sciences - X1A_W01 W06: knows the biological concepts and the chemistry of metabolic processes in the human body - X1A_W01, P1A_W01, P1A_W05. W09: knows the rules and principles in the field of occupational health and safety, basic concepts in the field of toxicology; legal acts concerning standards and requirements of chemical laboratories and legal regulations concerning hazardous substances and their storage and labeling - X1A_W06, X1A_W07

Learning outcomes - skills

U03: can use basic knowledge of chemical sciences in biomedical research - X1A_U01; X1A_U06 U09: can independently expand the knowledge of medical analytics and metabolomics - X1A_U07. U11: can describe and present chemical issues from various sources using a specialist language - X1A_U08; X1A_U09

Learning outcomes - social competencies

K01: Is focused on the continuous acquisition of new knowledge, skills and experiences; sees the need for continuous improvement and improvement of professional competences; knows the limitations of his own knowledge and understands the need for further education - X1A_K01; X1A_K05 K02: can organize work in a team and work effectively with a large amount of information, sees the relationships between phenomena and correctly draws conclusions using the principles of logic - X1A_K02 K06: understand the social aspects of the practical use of the acquired knowledge and skills - X1A_K07

Teaching methods

Lecture with discussion, multimedia presentations during lectures, instructions for laboratory exercises on the basis of which the student performs exercises in the laboratory, individual work of the listener with laboratory equipment and specialized analytical equipment under the supervision of the teacher, consultations with the teachers.

Prerequisites

The basic knowledge in an organic chemistry and a biochemistry. The basic knowledge in a sample preparation methods and an analytical chemistry.

Course coordinators

Term 2021/22Z:

Bogusław Buszewski
Magdalena Ligor

Term 2020/21:

Bogusław Buszewski
Magdalena Ligor

Term 2022/23Z:

Magdalena Ligor

Assessment criteria

The lecture ends with a grade. A longer written statement or presentation.
The final grade for the lecture will be determined on the basis of the obtained number of points according to the principle:
• for a satisfactory grade: obtaining more than 50% of the points by the student
• with a good mark: obtaining more than 70% of points by the student
• with a very good mark: obtaining more than 81% of points by the student.
The condition for completing the course is knowledge of the analytical procedures needed for the correct performance of the exercise-laboratory analysis, obtaining a positive mark from the test preceding the exercise, passing a written study for each exercise - W01, W02, K02

Bibliography

1. R.P. W. Scott , Tandem techniques, J. Wiley & Sons, New York 1998.
2. C.F. Poole, The Essence of Chromatography, Elsevier, Amsterdam 2003.
3. J. Pawliszyn [Ed.], Sampling and sample preparation for field and laboratory Elsevier Science B.V., Amsterdam 2002
4. J. Pawliszyn, Solid Phase Microextraction, Wiley & Sons, New York 1997.
5. B. Buszewski, E. Dziubakiewicz, M. Szumski [red.], Techniki elektromigracyjne: teoria i praktyka, Wydawnictwo MALAMUT, Warszawa 2012.
6. Z. Brzózka [red.]; Miniaturyzacja w analityce, Oficyna Wydawnicza Politechniki Warszawskiej, Warszawa 2005.
7. B. Ardrey, Liquid chromatography-mass spectrometry: an introduction, John Wiley & Sosns Inc., Chichester 2003.
8. W. Grajek, Przeciwutleniacze w żywności. Aspekty zdrowotne, technologiczne, molekularne i analityczne, Wydawnictwo Naukowo-Techniczne, Warszawa 2007.
9. G. Bartosz, Druga twarz tlenu, Wydawnictwo Naukowe PWN, Warszawa 2013.
10. Y. Surh-Joon, Oxidative stress, inflammation, and health, Wydawnictwo CRC Press, 2005.
11. S. Ball, Antyoksydanty w Medycynie i Zdrowiu Człowieka, wyd. I, Oficyna Wydawnicza Medyk, Warszawa 2001.
12. Baranowska I., Buszewski B., Bioanalityka. PWN, Warszawa 2020.
13. Buszewski B., Szultka M., Gadzała-Kopciuch R., Theory of extraction techniques, in: Comprehensive sampling and sample preparation, J. Pawliszyn (ed.) Academic Press, New York 2012, str. 243-256.
14. Buszewski B., Szultka M., Past, Present, and Future of Solid Phase Extraction: A Review. Critical Reviews in Analytical Chemistry, 42:3 (2012) 198-213.
15. Kealey D., Haines P.J., Krótkie wykłady, Chemia Analityczna. PWN, Warszawa 2006.
16. Ligor M., Ratiu I.A., Kiełbasa A., Al‐Suod H., Buszewski B., Extraction approaches used for the determination of biologically active compounds (cyclitols, polyphenols and saponins) isolated from plant material. Electrophoresis 39(15) (2018) 1860-1874.
17. Skoog D.A., West D.M., Holler J.F., Crouch S.R., Podstawy chemii analitycznej T. 2, przekład zbiorowy A. Hulanickiego [red.], Wydawnictwo Naukowe PWN, Warszawa 2007.
18. Thurman E. M., Mills M. S., Solid Phase Extraction. Principles and Practice. Wiley, Nowy Jork 1998.

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S1-CM-PW7a-Va in USOSweb