Fundamentals of Separation Methods 0600-S1-CM-PMS

Separation mechanisms, kinetic theory of chromatography, properties and selection of stationary phases, principles of selection of mobile phases, chromatographic apparatus, methods of sample preparation, methods of evaluation and criteria of column efficiency, qualitative and quantitative analysis.

Subjects:
1. Gas chromatography (GC) - interpretation of chromatograms, retention parameters, qualitative and quantitative analysis.
2. Total Organic Carbon (TOC) - calibration, measurement and methodology.
3. Isotachophoresis (ITP) appliction of selected cations in waters or infusion fluids.
4. Thin layer liquid chromatography (TLC). Separation of plant pigments (anthocyanins, chlorophyll).
5. High performance liquid chromatography (HPLC) - retention data, qualitative and quantitative analysis.
6. Method of separation by use of membrane.

Term 2022/23L:

None

Term 2023/24L:

None

Term 2024/25L:

None

Term 2025/26L:

None

Total student workload

Contact hours with teacher: - participation in lectures - 15 hrs - participation in laboratories – 45 hrs - consultations - 15 hrs Self-study hours: - preparation for lectures, writing essays/ papers/ projects, reading literature, preparation for test/examination - 15 hrs

Learning outcomes - knowledge

W01: uses and explains terminology specific to the subject in English and its equivalent in Polish W04: knows the theoretical and practical aspects of using chromatographic, electromigration and spectroscopic techniques in chemical analyzes

Learning outcomes - skills

K_U01: can analyze and solve chemical problems based on the acquired knowledge, independently prepare samples for analysis - X1A_U06, X1A_U07 K_U04: can plan an experiment and use the equipment used to carry out a specific research task X1A_U01; X1A_U02, X1A_U03 K_U09: can independently expand knowledge of a selected specialization - X1A_U07

Learning outcomes - social competencies

K_K02: the acquired skills will enable to independently develop analytical procedures and organize team work, taking different roles to perform the assigned tasks: X1A_K02 K_K03: can properly define priorities in order to plan and implement a specific task: X1A_K03, P1A_K04

Teaching methods

Lectures Laboratory experiments

Prerequisites

Basics of analytical chemistry. General issues in the field of chromatographic and electromigration techniques.

Course coordinators

Tomasz Ligor

Assessment criteria

To pass of laboratory experiments is the knowledge of analytical procedures required for the correct execution of exercises-laboratory analysis, a positive evaluation of the colloquium preceding the exercise include the written report for each experiments – W01, W02, K02
fail - 2 pts (≥50%)
satisfactory - 3 pts (50-60%)
satisfactory plus - 3,5 pts (61-65%)
good - 4 pts (66-75%)
good plus - 4,5 pts (76-80%)
very good - 5 pts (≤81%)

Practical placement

not applicable

Bibliography

I. Baranowska, B. Buszewski [red.], Bioanalityka w nauce i życiu - tom 1, PWN, Warszawa 2020, wyd. 1. ISBN: 978-83-01-21281-0.
I. Baranowska [red.]; Analiza śladowa. Zastosowania, Wydawnictwo MALAMUT, Warszawa 2013.
B. Buszewski, E. Dziubakiewicz, M. Szumski [red.], Techniki elektromigracyjne: teoria i praktyka, Wydawnictwo MALAMUT, Warszawa 2012.
Z. Brzózka [red.]; Miniaturyzacja w analityce, Oficyna Wydawnicza Politechniki Warszawskiej, Warszawa 2005.
J.R. Dean, Methods for environmental trace analysis, John Wiley & Sosns Inc., Chichester 2003.
B. Ardrey, Liquid chromatography-mass spectrometry: an introduction, John Wiley & Sosns Inc., Chichester 2003.
C. F. Poole, S. K. Poole, Chromatography today, Elsevier, Amsterdam 1991.
W. Rödel, G. Wölm. Chromatografia gazowa, Wydawnictwo Naukowe PWN, Warszawa 1992.
R.J. Hamilton, P.A. Sewell, Wysokosprawna chromatografia cieczowa, PWN, Warszawa 1982.
J. Namieśnik, Z. Jamrógiewicz (red), Fizykochemiczne metody kontroli zanieczyszczeń środowiska, WNT, Warszawa 1998.
P. Bocek, Analytical capillary isotachophoresis, Verlag Chemie, Wienheim 1987.

Term 2022/23L:

None

Term 2023/24L:

None

Term 2024/25L:

None

Term 2025/26L:

None

Additional information

Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system:

Description of 0600-S1-CM-PMS in USOSweb