Bioanalytics
0600-S1-CM-BioA
Introduction of general concepts of medicinal chemistry, drug chemistry, food chemistry - historical background, terminology. Characterisation of lipids, carbohydrates, proteins, enzymes, receptors, nucleic acids. Structure and properties of the cell. bioassays using appropriate test organisms, biosensors. Antibodies. Use of modern analytical tools with examples of selected chromatographic, electromigration, spectroscopic, radiometric, immunological techniques, mass analysers, ion detectors for the determination of biologically active substances. Modern methods for the analysis of bio-molecules. Specific methods of sampling and sample preparation for bioanalytical determinations. Performance criteria for research and clinical laboratories. Quality assurance systems. Accreditation and certification of laboratories related to bioanalytical testing. Analytical procedures and their validation. Statistical evaluation of results.
Total student workload
1. The workload related to activities requiring direct participation of academic teachers is:
- participation in lectures: 15 hours,
- participation in laboratories: 30 hours,
- participation in scientific and research consultations: 15 hours.
The total student workload related to the activities requiring direct participation of academic teachers is 60 hours, which corresponds to 2,4 ECTS credits. 2.
2. The balance of the student workload:
- participation in lectures: 15 hours,
- participation in laboratories: 30 hours,
- preparation for laboratories, preparation of papers: 4 hours,
- reading of indicated literature: 2 hours,
- participation in scientific and research consultations: 15 hours,
- preparation for the examination and the test: 5 hours
- preparation for the exam and examination: 4 hours.
The total amount of the student workload is 75 hours, which corresponds to 3 ECTS credits.
3. The workload related to the research:
- participation in lectures (including research methodology): 15 hours,
- realization of scientific research aspects within exercises: 15 hours,
- scientific research consultations: 1 hour,
- reading literature as part of the scientific research aspects covered: 10 hours,
- preparation for the pass in the research and scientific aspects for the subject pursued: 9 hours.
Total research-related working time: 81 hours, 2 ECTS.
4. Time required to prepare for and to participate in the assessment process:
- preparation for credit and colloquium - 6 hours,
- preparation for the examination and the examination - 10 hours + 1 hour = 11 hours.
The total time required for preparation and participation in the assessment process is 22 hours, which corresponds to 0.88 ECTS credits.
5. Time required for compulsory internship: not applicable.
Learning outcomes - knowledge
W1: has knowledge about theoretical basis and possibilities of using separation techniques with various detection methods for trace analysis of biologically active substances - X1A_W01
W09: he/she knows the regulations and rules concerning safety of toxic substances and basic concepts of their impact on the environment; legal acts concerning standards and requirements of chemical laboratories and regulations on toxic substances - X1A_W06, X1A_W07
W10: knows the basics of biochemistry and chemistry of metabolic processes, pollutants of various elements of the ecosystem and their impact on the human organism - P1A_W01.
Learning outcomes - skills
U01: is able to use basic knowledge of analytical and instrumental chemistry with their application to isolation and determination of toxic substances hazardous to the environment and man - X1A_U01; X1A_U06
U08: is able to describe and present bioanalytical issues from various sources using specialist language - X1A_U05; X1A_U08
U09: is able to independently expand the knowledge of bioanalytics, separation methods and methods of preparing environmental samples and biological material - X1A_U07.
Learning outcomes - social competencies
K01: is focused on continuous acquisition of new knowledge, skills and experience in the field of bioanalytics and modern instrumental analysis; knows the limitations of his/her own knowledge and understands the need for further education - X1A_K01; X1A_K05
K02: is able to organise work in a team and perceives the interdependencies between phenomena occurring in the natural environment, and correctly draws conclusions from their impact on living organisms - X1A_K02
K06: understands the social aspects of practical use of the acquired knowledge on the impact of pollution on living organisms - X1A_K03, X1A_K07
Teaching methods
Lecture:
- informative lecture,
- problem-based lecture with multimedia presentation.
Laboratories:
- exercise classes,
- work in teams and/or individually,
- measurement and analysis of results.
Observation/demonstration teaching methods
- display
Expository teaching methods
- problem-based lecture
- informative (conventional) lecture
Exploratory teaching methods
- classic problem-solving
- laboratory
- experimental
Type of course
compulsory course
Prerequisites
The student will have a basic knowledge of analytical chemistry, organic chemistry, biochemistry and medicinal chemistry before taking the Bioanalytical course. The student should have skills in analytical methods, precise weighing and measuring, preparing solutions, performing quantitative and qualitative analyses by classical methods as well as by instrumental methods, calculating the results of quantitative analysis.
Course coordinators
Assessment criteria
The prerequisite for passing the Bioanalytical course is: passing laboratory exercises getting above 50% in written colloquia and getting a positive mark in the final exam.
Lectures:
Pass grade on the basis of two written colloquia and a final examination in the form of a single-choice test (open and closed single-choice questions).
The points obtained from the examination are converted into marks according to the following scale:
Percentage of points - grade
81-100 % - Very good
76-80 % - Good plus
66-75 % - Good
61-65 % - Pass plus
51-60 % - Fair
0-51 % - Unsatisfactory
The final revision examination is held in the revision session.
Laboratories:
The pass mark for the laboratory exercises is obtained on the basis of active participation in the laboratory classes, during which the Student/Young person obtains credits for the work by performing the exercises according to the schedule, colloquia and papers from the theoretical and experimental parts, in order to obtain a pass mark for the laboratory exercises it is necessary to obtain a minimum of 50% of the sum of all the colloquia and to pass all the exercises performed.
Practical placement
Bibliography
Reference literature:
- J.M. Berg, Tymoczko J.L., Stryer L. Biochemistry. PWN, Warsaw 2011.
- D.B. Hames, Hooper N.M., Short lectures. Biochemistry, PWN, Warsaw 2010.
- J. Kączkowski, Basics of biochemistry. WN-T, Warszawa 2005.
- Szczepaniak W., Instrumental methods in chemical analysis, PWN Warszawa 2005.
- Modern analytical techniques, collective work edited by M. Jarosz, Oficyna Wyd. PW, Warsaw 2006
- Microbioanalytic, collective work, edited by Z. Brzózka, Oficyna Wydawnicza PW, Warsaw 2009
- S. R. Mikkelsen, E. Cortón, Bioanalytical Chemistry, Wiley-Interscience, Hoboken, New Jersey 2004
- H. Waldmann, P. Janning, Chemical Biology. A practical course, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 2004
- L. Huber, Good laboratory practice, PIOŚ, Warsaw 1997
- B. Buszewski, E. Dziubakiewicz, M. Szumski, Electromigration techniques: theory and practice, Malamut, Warsaw 2012.
- L. Klyszyko-Stefanowicz (ed.), Exercises in biochemistry. PWN, Warsaw 2011.
Complementary literature:
- P. Kafarski, P. Wieczorek, Laboratory Exercises in Bioorganic Chemistry, Opole University Publishing House, Opole 1997.
- J.B. Harborne - Biochemical ecology. PWN, Warsaw 1997.
- M. Siemiński - Środowiskowe zagrożenia zdrowia. PWN, Warsaw 2007.
- R. Czerpak - General Biochemistry. Part I. Structural biochemistry. FUW, Białystok 1993.
Additional information
Additional information (registration calendar, class conductors,
localization and schedules of classes), might be available in the USOSweb system: