Liquid chromatography 0600-PS-AOS-CC
Lecture:
Historical overview and the techniques development. Chromatographic methods classification. Adsorption vs. chromatography theory. Chromatographic terminology - basic terms. Separation process description. General problems of chromatographic analysis.
Modern TLC (Thin Layer Chromatography) – an introduction, apparatus, retention parameters, qualitative and quantitative analysis, application. HPLC apparatus (pumps, samplers, columns, detectors, thermostats, integrators, computer). Separation process optimization in LC (liquid chromatography): chromatographic system selection - mobile phase, stationary phase, detection, characteristics and properties. Qualitative and quantitative analysis in HPLC. Interpretation of chromatograms. Testing and columns quality evaluation for HPLC, UHPLC and microHPLC. Gel chromatography. Preparative Chromatography (column and TLC). Ultra high performance liquid chromatography and its application to the biological and environmental samples studies. Trace analysis. Calibration. Certified reference materials. Sample preparation techniques and procedures. Natural products analysis. Group analysis. Hyphenated techniques. Automatic control systems. Validation. Standards and European Union directives - quality assurance of provided analysis.
Become familiar with the one of most popular and commonly used analytical techniques - liquid chromatography, and its place in analytics. Sample preparation for liquid chromatography, apparatus and detectors, analytical methods and instrumental measurement errors, validation.
Laboratory classes:
Thin Layer Chromatography (TLC: analytical, preparative and under pressure) – separation background (absorbents, eluents and chamber types, ways of developing and visualizing chromatograms, qualitative and quantitative analysis).
High performance liquid chromatography (HPLC) - retention data, qualitative and quantitative analysis - the plate theory based on the thermodynamics, resolution, selectivity, detection systems, qualitative and quantitative analysis in separation techniques.
Purnell’s equation. Knox - Bristow model. Qualitative and quantitative analysis in HPLC. Quality assessment of LC columns for e.g. HPLC. Selection of UV detection conditions. Interpretation of chromatograms. Selection of mobile phase composition in normal and reversed-phase system. Selection of mobile phase composition in RP HPLC system in isocratic and gradient elution by using ChromSword software. Isoeluotropic eluents. Polarity of solvents. Become familiar with the selection terms of different detectors properties. Detectors evaluation. Automation and robotics. Miniaturization.
These classes are leading according to the good laboratory practice (GLP) rules and principles.
Total student workload
Learning outcomes - knowledge
Learning outcomes - skills
Learning outcomes - social competencies
Teaching methods
Type of course
Prerequisites
Course coordinators
Learning outcomes
Student - graduate student:
1. identifies and distinguish between issues related to the classes topic;
2. are able to find and use the required literature in English and Polish;
3. are using, applying and explaining the subject terminology related
to the study issues (not using laboratory slang) in English
and its counterparts in Polish;
4. are applying in practice the theoretical knowledge how to operate
by the apparatus and small laboratory equipment in a correct and proper way as well. Making self measurements based on the knowledge he/she gained;
5. preparing their own preparations for analysis, creating analytical procedures and standard procedures;
6. independently examines, interprets and calculates the results obtained
in the laboratory. Applying the appropriate analytical procedures. Preparing writing research reports and notes, which may be the useful for issuing the scientific publications;
7. compares, explains and describes obtained results in comparison with the available standards and literature. Predicting behavior and probable scenarios in the laboratory during sample preparation as well as the same study (e.g. principles of health and safety in the workplace).
Assessment criteria
The pass mark class is attendance at lectures and active participation, knowledge of analytical procedures is required for proper implementation
of the exercise-laboratory analysis.
Course assessment is based on the frequency and participation in the classes as well as receiving a positive evaluation at an oral, final exam.
Practical placement
not provided
Bibliography
B. Buszewski, Chromatografia i techniki pokrewne w chemii środowiska, UMK, Toruń, 1998,
W. Szczepaniak, Metody instrumentalne w analizie chemicznej, PWN, Warszawa, 2004;
J. Garaj, D. Bustin, Z. Hladky, Chemia analityczna, Alfa, SNTL Bratislava, 1989;
J. Namieśnik (red. nauk), Metody instrumentalne w kontroli zanieczyszczeń środowiska, WNT Warszawa, 1998;
Praca zbiorowa, Nowe horyzonty i wyzwania w analityce i monitoringu środowiskowy, CEEAM Gdańsk 2003
Praca zbiorowa, Miniaturyzacja w analityce, WPW Warszawa 2005
Praca zbiorowa, Nowoczesne techniki analityczne, WPW Warszawa 2006
Harris, Quantitative & Qualitative Chemical Anaylysis, Freeman, New York 1999
C.F. Pool &S. H. Pool, Chromatogrpahy today, Elsevier, Amsterdam 1992
S. Mitra Sample preparation techniques in analytical chemistry, Wiley, New Jersey 2003
R.P. Scott, Tandem techniques, J. Wiley & Sons, New York 1998
Additional information
Additional information (registration calendar, class conductors, localization and schedules of classes), might be available in the USOSweb system: